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-Structure paper
タイトル | Structural Insights into Iron Ions Accumulation in Dps Nanocage. |
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ジャーナル・号・ページ | Int J Mol Sci, Vol. 23, Issue 10, Year 2022 |
掲載日 | 2022年5月10日 |
著者 | Yury Chesnokov / Andrey Mozhaev / Roman Kamyshinsky / Alexander Gordienko / Liubov Dadinova / |
PubMed 要旨 | Dps (DNA-binding protein from starved cells) is well known for the structural protection of bacterial DNA by the formation of highly ordered intracellular assemblies under stress conditions. ...Dps (DNA-binding protein from starved cells) is well known for the structural protection of bacterial DNA by the formation of highly ordered intracellular assemblies under stress conditions. Moreover, this ferritin-like protein can perform fast oxidation of ferrous ions and subsequently accumulate clusters of ferric ions in its nanocages, thus providing the bacterium with physical and chemical protection. Here, cryo-electron microscopy was used to study the accumulation of iron ions in the nanocage of a Dps protein from . We demonstrate that Fe concentration in the solution and incubation time have an insignificant effect on the volume and the morphology of iron minerals formed in Dps nanocages. However, an increase in the Fe level leads to an increase in the proportion of larger clusters and the clusters themselves are composed of discrete ~1-1.5 nm subunits. |
リンク | Int J Mol Sci / PubMed:35628121 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 2.59 - 14.5 Å |
構造データ | EMDB-14943: Structure of DPS EMDB-14948: Dps nanocage with the "small circle" mineral core EMDB-14949: Dps nanocage with the "dumbbell" mineral core EMDB-14950: Dps nanocage with a medium mineral core EMDB-14951: Dps nanocage with a large mineral core EMDB-14952: Dps nanocage with the symmetrical hollow mineral core |
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