+検索条件
-Structure paper
タイトル | Three-dimensional structure of vertebrate cardiac muscle myosin filaments. |
---|---|
ジャーナル・号・ページ | Proc Natl Acad Sci U S A, Vol. 105, Issue 7, Page 2386-2390, Year 2008 |
掲載日 | 2008年2月19日 |
著者 | Maria E Zoghbi / John L Woodhead / Richard L Moss / Roger Craig / |
PubMed 要旨 | Contraction of the heart results from interaction of the myosin and actin filaments. Cardiac myosin filaments consist of the molecular motor myosin II, the sarcomeric template protein, titin, and the ...Contraction of the heart results from interaction of the myosin and actin filaments. Cardiac myosin filaments consist of the molecular motor myosin II, the sarcomeric template protein, titin, and the cardiac modulatory protein, myosin binding protein C (MyBP-C). Inherited hypertrophic cardiomyopathy (HCM) is a disease caused mainly by mutations in these proteins. The structure of cardiac myosin filaments and the alterations caused by HCM mutations are unknown. We have used electron microscopy and image analysis to determine the three-dimensional structure of myosin filaments from wild-type mouse cardiac muscle and from a MyBP-C knockout model for HCM. Three-dimensional reconstruction of the wild-type filament reveals the conformation of the myosin heads and the organization of titin and MyBP-C at 4 nm resolution. Myosin heads appear to interact with each other intramolecularly, as in off-state smooth muscle myosin [Wendt T, Taylor D, Trybus KM, Taylor K (2001) Proc Natl Acad Sci USA 98:4361-4366], suggesting that all relaxed muscle myosin IIs may adopt this conformation. Titin domains run in an elongated strand along the filament surface, where they appear to interact with part of MyBP-C and with the myosin backbone. In the knockout filament, some of the myosin head interactions are disrupted, suggesting that MyBP-C is important for normal relaxation of the filament. These observations provide key insights into the role of the myosin filament in cardiac contraction, assembly, and disease. The techniques we have developed should be useful in studying the structural basis of other myosin-related HCM diseases. |
リンク | Proc Natl Acad Sci U S A / PubMed:18252826 / PubMed Central |
手法 | EM (らせん対称) |
解像度 | 32.0 Å |
構造データ | EMDB-1465: |
由来 |
|