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-Structure paper
タイトル | Mitoribosome structure with cofactors and modifications reveals mechanism of ligand binding and interactions with L1 stalk. |
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ジャーナル・号・ページ | Nat Commun, Vol. 15, Issue 1, Page 4272, Year 2024 |
掲載日 | 2024年5月20日 |
著者 | Vivek Singh / Yuzuru Itoh / Samuel Del'Olio / Asem Hassan / Andreas Naschberger / Rasmus Kock Flygaard / Yuko Nobe / Keiichi Izumikawa / Shintaro Aibara / Juni Andréll / Paul C Whitford / Antoni Barrientos / Masato Taoka / Alexey Amunts / |
PubMed 要旨 | The mitoribosome translates mitochondrial mRNAs and regulates energy conversion that is a signature of aerobic life forms. We present a 2.2 Å resolution structure of human mitoribosome together ...The mitoribosome translates mitochondrial mRNAs and regulates energy conversion that is a signature of aerobic life forms. We present a 2.2 Å resolution structure of human mitoribosome together with validated mitoribosomal RNA (rRNA) modifications, including aminoacylated CP-tRNA. The structure shows how mitoribosomal proteins stabilise binding of mRNA and tRNA helping to align it in the decoding center, whereas the GDP-bound mS29 stabilizes intersubunit communication. Comparison between different states, with respect to tRNA position, allowed us to characterize a non-canonical L1 stalk, and molecular dynamics simulations revealed how it facilitates tRNA transitions in a way that does not require interactions with rRNA. We also report functionally important polyamines that are depleted when cells are subjected to an antibiotic treatment. The structural, biochemical, and computational data illuminate the principal functional components of the translation mechanism in mitochondria and provide a description of the structure and function of the human mitoribosome. |
リンク | Nat Commun / PubMed:38769321 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 2.21 - 2.98 Å |
構造データ | EMDB-13980: Human mitochondrial ribosome at 2.2 A resolution EMDB-13981, PDB-7qi5: EMDB-13982, PDB-7qi6: |
化合物 | ChemComp-NAD: ChemComp-SPM: ChemComp-SPD: ChemComp-MG: ChemComp-K: ChemComp-ZN: ChemComp-FES: ChemComp-ATP: ChemComp-GDP: ChemComp-PUT: ChemComp-VAL: ChemComp-HOH: |
由来 |
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キーワード | RIBOSOME / mitochondrial translation / tRNA / mRNA / 2Fe-2S clusters / polyamines / rRNA modifications / post-translation modifications / cryo EM |