EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural snapshots of La Crosse virus polymerase reveal the mechanisms underlying Peribunyaviridae replication and transcription.
ジャーナル・号・ページ	Nat Commun, Vol. 13, Issue 1, Page 902, Year 2022
掲載日	2022年2月16日
著者	Benoît Arragain / Quentin Durieux Trouilleton / Florence Baudin / Jan Provaznik / Nayara Azevedo / Stephen Cusack / Guy Schoehn / Hélène Malet /
PubMed 要旨	Segmented negative-strand RNA bunyaviruses encode a multi-functional polymerase that performs genome replication and transcription. Here, we establish conditions for in vitro activity of La Crosse ...Segmented negative-strand RNA bunyaviruses encode a multi-functional polymerase that performs genome replication and transcription. Here, we establish conditions for in vitro activity of La Crosse virus polymerase and visualize its conformational dynamics by cryo-electron microscopy, unveiling the precise molecular mechanics underlying its essential activities. We find that replication initiation is coupled to distal duplex promoter formation, endonuclease movement, prime-and-realign loop extension and closure of the polymerase core that direct the template towards the active site. Transcription initiation depends on C-terminal region closure and endonuclease movements that prompt primer cleavage prior to primer entry in the active site. Product realignment after priming, observed in replication and transcription, is triggered by the prime-and-realign loop. Switch to elongation results in polymerase reorganization and core region opening to facilitate template-product duplex formation in the active site cavity. The uncovered detailed mechanics should be helpful for the future design of antivirals counteracting bunyaviral life threatening pathogens.
リンク	Nat Commun / PubMed:35173159 / PubMed Central
手法	EM (単粒子)
解像度	2.8 - 3.9 Å
構造データ	13038 7ori EMDB-13038, PDB-7ori: La Crosse virus polymerase at replication late-elongation stage 手法: EM (単粒子) / 解像度: 3.9 Å 13039 7orj EMDB-13039, PDB-7orj: La Crosse virus polymerase at transcription capped RNA cleavage stage 手法: EM (単粒子) / 解像度: 3.9 Å 13040 7ork EMDB-13040, PDB-7ork: La Crosse virus polymerase in transcription mode with cleaved capped RNA entering the polymerase active site 手法: EM (単粒子) / 解像度: 3.1 Å 13041 7orl EMDB-13041, PDB-7orl: La Crosse virus polymerase at transcription initiation stage 手法: EM (単粒子) / 解像度: 3.6 Å 13042 7orm EMDB-13042, PDB-7orm: La Crosse virus polymerase at transcription early-elongation stage 手法: EM (単粒子) / 解像度: 3.3 Å 13043 7orn EMDB-13043, PDB-7orn: La Crosse virus polymerase at replication initiation stage 手法: EM (単粒子) / 解像度: 2.8 Å 13044 7oro EMDB-13044, PDB-7oro: La Crosse virus polymerase at replication early-elongation stage 手法: EM (単粒子) / 解像度: 2.9 Å
化合物	ChemComp-ZN: Unknown entry ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-POP: PYROPHOSPHATE 2- ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM ChemComp-HOH: WATER ChemComp-GTG*: 7-METHYL-GUANOSINE-5'-TRIPHOSPHATE-5'-GUANOSINE / 7-メチルGp₃G
由来	la crosse orthobunyavirus (ウイルス) bunyavirus la crosse (ウイルス) la crosse virus (ウイルス) homo sapiens (ヒト)
キーワード	VIRAL PROTEIN / RNA-dependent RNA polymerase