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-Structure paper
タイトル | Correlative multi-scale cryo-imaging unveils SARS-CoV-2 assembly and egress. |
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ジャーナル・号・ページ | Nat Commun, Vol. 12, Issue 1, Page 4629, Year 2021 |
掲載日 | 2021年7月30日 |
著者 | Luiza Mendonça / Andrew Howe / James B Gilchrist / Yuewen Sheng / Dapeng Sun / Michael L Knight / Laura C Zanetti-Domingues / Benji Bateman / Anna-Sophia Krebs / Long Chen / Julika Radecke / Vivian D Li / Tao Ni / Ilias Kounatidis / Mohamed A Koronfel / Marta Szynkiewicz / Maria Harkiolaki / Marisa L Martin-Fernandez / William James / Peijun Zhang / |
PubMed 要旨 | Since the outbreak of the SARS-CoV-2 pandemic, there have been intense structural studies on purified viral components and inactivated viruses. However, structural and ultrastructural evidence on how ...Since the outbreak of the SARS-CoV-2 pandemic, there have been intense structural studies on purified viral components and inactivated viruses. However, structural and ultrastructural evidence on how the SARS-CoV-2 infection progresses in the native cellular context is scarce, and there is a lack of comprehensive knowledge on the SARS-CoV-2 replicative cycle. To correlate cytopathic events induced by SARS-CoV-2 with virus replication processes in frozen-hydrated cells, we established a unique multi-modal, multi-scale cryo-correlative platform to image SARS-CoV-2 infection in Vero cells. This platform combines serial cryoFIB/SEM volume imaging and soft X-ray cryo-tomography with cell lamellae-based cryo-electron tomography (cryoET) and subtomogram averaging. Here we report critical SARS-CoV-2 structural events - e.g. viral RNA transport portals, virus assembly intermediates, virus egress pathway, and native virus spike structures, in the context of whole-cell volumes revealing drastic cytppathic changes. This integrated approach allows a holistic view of SARS-CoV-2 infection, from the whole cell to individual molecules. |
リンク | Nat Commun / PubMed:34330917 / PubMed Central |
手法 | EM (サブトモグラム平均) |
解像度 | 10.6 - 16.0 Å |
構造データ | EMDB-12991: EMDB-12992: |