EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structure of RNA polymerase II pre-initiation complex at 2.9 Å defines initial DNA opening.
ジャーナル・号・ページ	Cell, Vol. 184, Issue 15, Page 4064-4072.e28, Year 2021
掲載日	2021年7月22日
著者	Sandra Schilbach / Shintaro Aibara / Christian Dienemann / Frauke Grabbe / Patrick Cramer /
PubMed 要旨	Transcription initiation requires assembly of the RNA polymerase II (Pol II) pre-initiation complex (PIC) and opening of promoter DNA. Here, we present the long-sought high-resolution structure of ...Transcription initiation requires assembly of the RNA polymerase II (Pol II) pre-initiation complex (PIC) and opening of promoter DNA. Here, we present the long-sought high-resolution structure of the yeast PIC and define the mechanism of initial DNA opening. We trap the PIC in an intermediate state that contains half a turn of open DNA located 30-35 base pairs downstream of the TATA box. The initially opened DNA region is flanked and stabilized by the polymerase "clamp head loop" and the TFIIF "charged region" that both contribute to promoter-initiated transcription. TFIIE facilitates initiation by buttressing the clamp head loop and by regulating the TFIIH translocase. The initial DNA bubble is then extended in the upstream direction, leading to the open promoter complex and enabling start-site scanning and RNA synthesis. This unique mechanism of DNA opening may permit more intricate regulation than in the Pol I and Pol III systems.
リンク	Cell / PubMed:34133942
手法	EM (単粒子)
解像度	2.6 - 3.6 Å
構造データ	12719 7o4i EMDB-12719, PDB-7o4i: Yeast RNA polymerase II transcription pre-initiation complex with initial transcription bubble 手法: EM (単粒子) / 解像度: 3.2 Å 12720 7o4j EMDB-12720, PDB-7o4j: Yeast RNA polymerase II transcription pre-initiation complex (consensus) 手法: EM (単粒子) / 解像度: 2.9 Å 12721 7o4k EMDB-12721, PDB-7o4k: Yeast TFIIH in the contracted state within the pre-initiation complex 手法: EM (単粒子) / 解像度: 3.6 Å 12722 7o4l EMDB-12722, PDB-7o4l: Yeast TFIIH in the expanded state within the pre-initiation complex 手法: EM (単粒子) / 解像度: 3.4 Å EMDB-12723: Focused refinement of the upstream complex region of a RNA polymerase II transcription pre-initiation assembly 手法: EM (単粒子) / 解像度: 3.2 Å EMDB-12724: Focused refinement of RNA polymerase II (10-subunit) - TFIIB - TFIIF region of a transcription pre-initiation assembly 手法: EM (単粒子) / 解像度: 2.8 Å EMDB-12725: Focused refinement of RNA polymerase II (10-subunit) - TFIIB region of a transcription pre-initiation assembly 手法: EM (単粒子) / 解像度: 2.6 Å 12743 7o72 EMDB-12743, PDB-7o72: Yeast RNA polymerase II transcription pre-initiation complex with closed promoter DNA 手法: EM (単粒子) / 解像度: 3.4 Å 12744 7o73 EMDB-12744, PDB-7o73: Yeast RNA polymerase II transcription pre-initiation complex with closed distorted promoter DNA 手法: EM (単粒子) / 解像度: 3.4 Å 12745 7o75 EMDB-12745, PDB-7o75: Yeast RNA polymerase II transcription pre-initiation complex with open promoter DNA 手法: EM (単粒子) / 解像度: 3.2 Å
化合物	ChemComp-SF4: IRON/SULFUR CLUSTER / 鉄・硫黄クラスター ChemComp-ZN: Unknown entry ChemComp-BEF: BERYLLIUM TRIFLUORIDE ION ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子*YM / アデノシン二リン酸
由来	saccharomyces cerevisiae (strain atcc 204508 / s288c) (パン酵母) synthetic construct (人工物) Baker's yeast (パン酵母) saccharomyces cerevisiae s288c (パン酵母) saccharomyces cerevisiae yjm984 (パン酵母)
キーワード	TRANSCRIPTION (転写 (生物学)) / Pre-initiation complex