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-Structure paper
タイトル | Mycobacterium tuberculosis ferritin: a suitable workhorse protein for cryo-EM development. |
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ジャーナル・号・ページ | Acta Crystallogr D Struct Biol, Vol. 77, Issue Pt 8, Page 1077-1083, Year 2021 |
掲載日 | 2021年8月1日 |
著者 | Abril Gijsbers / Yue Zhang / Ye Gao / Peter J Peters / Raimond B G Ravelli / |
PubMed 要旨 | The use of cryo-EM continues to expand worldwide and calls for good-quality standard proteins with simple protocols for their production. Here, a straightforward expression and purification protocol ...The use of cryo-EM continues to expand worldwide and calls for good-quality standard proteins with simple protocols for their production. Here, a straightforward expression and purification protocol is presented that provides an apoferritin, bacterioferritin B (BfrB), from Mycobacterium tuberculosis with high yield and purity. A 2.12 Å resolution cryo-EM structure of BfrB is reported, showing the typical cage-like oligomer constituting of 24 monomers related by 432 symmetry. However, it also contains a unique C-terminal extension (164-181), which loops into the cage region of the shell and provides extra stability to the protein. Part of this region was ambiguous in previous crystal structures but could be built within the cryo-EM map. These findings and this protocol could serve the growing cryo-EM community in characterizing and pushing the limits of their electron microscopes and workflows. |
リンク | Acta Crystallogr D Struct Biol / PubMed:34342280 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 2.1 Å |
構造データ | EMDB-12738, PDB-7o6e: |
化合物 | ChemComp-HOH: |
由来 |
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キーワード | METAL TRANSPORT / Iron storage; Ferroxidase; Bacterial Ferritin; Octahedral symmetry. |