EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Molecular interplay of an assembly machinery for nitrous oxide reductase.
ジャーナル・号・ページ	Nature, Vol. 608, Issue 7923, Page 626-631, Year 2022
掲載日	2022年7月27日
著者	Christoph Müller / Lin Zhang / Sara Zipfel / Annika Topitsch / Marleen Lutz / Johannes Eckert / Benedikt Prasser / Mohamed Chami / Wei Lü / Juan Du / Oliver Einsle /
PubMed 要旨	Emissions of the critical ozone-depleting and greenhouse gas nitrous oxide (NO) from soils and industrial processes have increased considerably over the last decades. As the final step of bacterial ...Emissions of the critical ozone-depleting and greenhouse gas nitrous oxide (NO) from soils and industrial processes have increased considerably over the last decades. As the final step of bacterial denitrification, NO is reduced to chemically inert N (refs. ) in a reaction that is catalysed by the copper-dependent nitrous oxide reductase (NOR) (ref. ). The assembly of its unique [4Cu:2S] active site cluster Cu requires both the ATP-binding-cassette (ABC) complex NosDFY and the membrane-anchored copper chaperone NosL (refs. ). Here we report cryo-electron microscopy structures of Pseudomonas stutzeri NosDFY and its complexes with NosL and NOR, respectively. We find that the periplasmic NosD protein contains a binding site for a Cu ion and interacts specifically with NosL in its nucleotide-free state, whereas its binding to NOR requires a conformational change that is triggered by ATP binding. Mutually exclusive structures of NosDFY in complex with NosL and with NOR reveal a sequential metal-trafficking and assembly pathway for a highly complex copper site. Within this pathway, NosDFY acts as a mechanical energy transducer rather than as a transporter. It links ATP hydrolysis in the cytoplasm to a conformational transition of the NosD subunit in the periplasm, which is required for NosDFY to switch its interaction partner so that copper ions are handed over from the chaperone NosL to the enzyme NOR.
リンク	Nature / PubMed:35896743
手法	EM (単粒子)
解像度	3.04 - 4.5 Å
構造データ	12683 7o0y EMDB-12683, PDB-7o0y: ABC transporter NosDFY, nucleotide-free in GDN 手法: EM (単粒子) / 解像度: 3.3 Å 12684 7o0z EMDB-12684, PDB-7o0z: ABC transporter NosFY, nucleotide-free in GDN 手法: EM (単粒子) / 解像度: 3.7 Å 12685 7o10 EMDB-12685, PDB-7o10: ABC transporter NosDFY, nucleotide-free in GDN, R-domain 2 手法: EM (単粒子) / 解像度: 3.6 Å 12686 7o11 EMDB-12686, PDB-7o11: ABC transporter NosDFY, nucleotide-free in GDN, R-domain 1 手法: EM (単粒子) / 解像度: 3.7 Å 12687 7o12 EMDB-12687, PDB-7o12: ABC transporter NosDFY, AMPPNP-bound in GDN 手法: EM (単粒子) / 解像度: 3.7 Å 12688 7o13 EMDB-12688, PDB-7o13: ABC transporter NosDFY, nucleotide-free in lipid nanodisc 手法: EM (単粒子) / 解像度: 3.6 Å 12689 7o14 EMDB-12689, PDB-7o14: ABC transporter NosDFY, nucleotide-free in lipid nanodisc, R-domain 1 手法: EM (単粒子) 12690 7o15 EMDB-12690, PDB-7o15: ABC transporter NosDFY, nucleotide-free in lipid nanodisc, R-domain 2 手法: EM (単粒子) 12691 7o16 EMDB-12691, PDB-7o16: ABC transporter NosDFY, nucleotide-free in lipid nanodisc, R-domain 3 手法: EM (単粒子) 12692 7o17 EMDB-12692, PDB-7o17: ABC transporter NosDFY E154Q, ATP-bound in lipid nanodisc 手法: EM (単粒子) / 解像度: 4.5 Å 13049 7osf EMDB-13049, PDB-7osf: ABC Transporter complex NosDFYL, R-domain 1 手法: EM (単粒子) / 解像度: 3.8 Å 13050 7osg EMDB-13050, PDB-7osg: ABC Transporter complex NosDFYL, consensus refinement 手法: EM (単粒子) / 解像度: 3.3 Å 13051 7osh EMDB-13051, PDB-7osh: ABC Transporter complex NosDFYL, R-domain 2 手法: EM (単粒子) / 解像度: 3.8 Å 13052 7osi EMDB-13052, PDB-7osi: ABC Transporter complex NosDFYL, R-domain 3 手法: EM (単粒子) / 解像度: 3.8 Å 13053 7osj EMDB-13053, PDB-7osj: ABC Transporter complex NosDFYL, membrane anchor 手法: EM (単粒子) / 解像度: 3.8 Å 13885 7qba EMDB-13885, PDB-7qba: CryoEM structure of the ABC transporter NosDFY complexed with nitrous oxide reductase NosZ 手法: EM (単粒子) / 解像度: 3.78 Å 14813 7znq EMDB-14813, PDB-7znq: ABC transporter complex NosDFYL in GDN 手法: EM (単粒子) / 解像度: 3.04 Å
化合物	ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-HOH: WATER ChemComp-ANP: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / AMP-PNP / AMP-PNP, エネルギー貯蔵分子類似体YM ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM ChemComp-CU: COPPER (II) ION ChemComp-ZN: Unknown entry ChemComp-CA: Unknown entry / カルシウムジカチオン
由来	pseudomonas stutzeri atcc 14405 = ccug 16156 (バクテリア) pseudomonas stutzeri (バクテリア)
キーワード	MEMBRANE PROTEIN / ABC Transporter complex / nucleotide-free / ABC Transporter / AMPPNP-bound / ATP-bound / metal-binding / ATP-free / metal binding protein / nitrous oxide reductase / ATP-binding-cassette transporter complex NosDFYL