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-Structure paper
タイトル | Mechanistic insight into bacterial entrapment by septin cage reconstitution. |
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ジャーナル・号・ページ | Nat Commun, Vol. 12, Issue 1, Page 4511, Year 2021 |
掲載日 | 2021年7月23日 |
著者 | Damián Lobato-Márquez / Jingwei Xu / Gizem Özbaykal Güler / Adaobi Ojiakor / Martin Pilhofer / Serge Mostowy / |
PubMed 要旨 | Septins are cytoskeletal proteins that assemble into hetero-oligomeric complexes and sense micron-scale membrane curvature. During infection with Shigella flexneri, an invasive enteropathogen, ...Septins are cytoskeletal proteins that assemble into hetero-oligomeric complexes and sense micron-scale membrane curvature. During infection with Shigella flexneri, an invasive enteropathogen, septins restrict actin tail formation by entrapping bacteria in cage-like structures. Here, we reconstitute septin cages in vitro using purified recombinant septin complexes (SEPT2-SEPT6-SEPT7), and study how these recognize bacterial cells and assemble on their surface. We show that septin complexes recognize the pole of growing Shigella cells. An amphipathic helix domain in human SEPT6 enables septins to sense positively curved membranes and entrap bacterial cells. Shigella strains lacking lipopolysaccharide components are more efficiently entrapped in septin cages. Finally, cryo-electron tomography of in vitro cages reveals how septins assemble as filaments on the bacterial cell surface. |
リンク | Nat Commun / PubMed:34301939 / PubMed Central |
手法 | EM (トモグラフィー) |
構造データ | EMDB-12562: EMDB-12565: EMDB-12571: EMDB-12578: EMDB-12579: EMDB-12580: |
由来 |
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