EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Higher-order structures of the foot-and-mouth disease virus RNA-dependent RNA polymerase required for genome replication.
ジャーナル・号・ページ	Commun Biol, Vol. 5, Issue 1, Page 61, Year 2022
掲載日	2022年1月17日
著者	Eleni-Anna Loundras / James Streetley / Morgan R Herod / Rebecca Thompson / Mark Harris / David Bhella / Nicola J Stonehouse /
PubMed 要旨	Replication of many positive-sense RNA viruses occurs within intracellular membrane-associated compartments. These are thought to provide a favourable environment for replication to occur, ...Replication of many positive-sense RNA viruses occurs within intracellular membrane-associated compartments. These are thought to provide a favourable environment for replication to occur, concentrating essential viral structural and nonstructural components, as well as protecting these components from host-cell pathogen recognition and innate immune responses. However, the details of the molecular interactions and dynamics within these structures is very limited. One of the key components of the replication machinery is the RNA-dependent RNA polymerase, RdRp. This enzyme has been shown to form higher-order fibrils in vitro. Here, using the RdRp from foot-and-mouth disease virus (termed 3D), we report fibril structures, solved at ~7-9 Å resolution by cryo-EM, revealing multiple conformations of a flexible assembly. Fitting high-resolution coordinates led to the definition of potential intermolecular interactions. We employed mutagenesis using a sub-genomic replicon system to probe the importance of these interactions for replication. We use these data to propose models for the role of higher-order 3D complexes as a dynamic scaffold within which RNA replication can occur.
リンク	Commun Biol / PubMed:35039618 / PubMed Central
手法	EM (らせん対称)
解像度	7.3 - 9.5 Å
構造データ	EMDB-12093: Higher-order structures of the foot-and-mouth disease virus RNA-dependent RNA polymerase required for dynamic inter-molecular interactions involved in viral genome replication 手法: EM (らせん対称) / 解像度: 7.3 Å EMDB-12094: Higher-order structures of the foot-and-mouth disease virus RNA-dependent RNA polymerase required for dynamic inter-molecular interactions involved in viral genome replication 手法: EM (らせん対称) / 解像度: 9.5 Å EMDB-12096: Higher-order structures of the foot-and-mouth disease virus RNA-dependent RNA polymerase required for dynamic inter-molecular interactions involved in viral genome replication 手法: EM (らせん対称) / 解像度: 8.5 Å EMDB-12097: Higher-order structures of the foot-and-mouth disease virus RNA-dependent RNA polymerase required for dynamic inter-molecular interactions involved in viral genome replication 手法: EM (らせん対称) / 解像度: 7.7 Å EMDB-12098: Higher-order structures of the foot-and-mouth disease virus RNA-dependent RNA polymerase required for dynamic inter-molecular interactions involved in viral genome replication 手法: EM (らせん対称) / 解像度: 7.6 Å EMDB-12099: Higher-order structures of the foot-and-mouth disease virus RNA-dependent RNA polymerase required for dynamic inter-molecular interactions involved in viral genome replication 手法: EM (らせん対称) / 解像度: 7.6 Å EMDB-12100: Higher-order structures of the foot-and-mouth disease virus RNA-dependent RNA polymerase required for dynamic inter-molecular interactions involved in viral genome replication 手法: EM (らせん対称) / 解像度: 7.3 Å EMDB-12101: Higher-order structures of the foot-and-mouth disease virus RNA-dependent RNA polymerase required for dynamic inter-molecular interactions involved in viral genome replication 手法: EM (らせん対称) / 解像度: 7.5 Å EMDB-12102: Higher-order structures of the foot-and-mouth disease virus RNA-dependent RNA polymerase required for dynamic inter-molecular interactions involved in viral genome replication 手法: EM (らせん対称) / 解像度: 7.3 Å
由来	Foot-and-mouth disease virus (口蹄疫ウイルス)