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-Structure paper
タイトル | Capsid opening enables genome release of iflaviruses. |
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ジャーナル・号・ページ | Sci Adv, Vol. 7, Issue 1, Year 2021 |
掲載日 | 2021年1月1日 |
著者 | Karel Škubník / Lukáš Sukeník / David Buchta / Tibor Füzik / Michaela Procházková / Jana Moravcová / Lenka Šmerdová / Antonín Přidal / Robert Vácha / Pavel Plevka / |
PubMed 要旨 | The family Iflaviridae includes economically important viruses of the western honeybee such as deformed wing virus, slow bee paralysis virus, and sacbrood virus. Iflaviruses have nonenveloped virions ...The family Iflaviridae includes economically important viruses of the western honeybee such as deformed wing virus, slow bee paralysis virus, and sacbrood virus. Iflaviruses have nonenveloped virions and capsids organized with icosahedral symmetry. The genome release of iflaviruses can be induced in vitro by exposure to acidic pH, implying that they enter cells by endocytosis. Genome release intermediates of iflaviruses have not been structurally characterized. Here, we show that conformational changes and expansion of iflavirus RNA genomes, which are induced by acidic pH, trigger the opening of iflavirus particles. Capsids of slow bee paralysis virus and sacbrood virus crack into pieces. In contrast, capsids of deformed wing virus are more flexible and open like flowers to release their genomes. The large openings in iflavirus particles enable the fast exit of genomes from capsids, which decreases the probability of genome degradation by the RNases present in endosomes. |
リンク | Sci Adv / PubMed:33523856 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 4.8 - 27.0 Å |
構造データ | EMDB-11699: EMDB-11703: EMDB-11704: EMDB-11705: EMDB-11706: EMDB-11709: EMDB-11711: EMDB-11714: EMDB-11716: EMDB-11815, PDB-7al3: |
由来 |
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キーワード | VIRUS / DWV / acidic pH / genome-containing |