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-Structure paper
タイトル | Desmosome architecture derived from molecular dynamics simulations and cryo-electron tomography. |
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ジャーナル・号・ページ | Proc Natl Acad Sci U S A, Vol. 117, Issue 44, Page 27132-27140, Year 2020 |
掲載日 | 2020年11月3日 |
著者 | Mateusz Sikora / Utz H Ermel / Anna Seybold / Michael Kunz / Giulia Calloni / Julian Reitz / R Martin Vabulas / Gerhard Hummer / Achilleas S Frangakis / |
PubMed 要旨 | Desmosomes are cell-cell junctions that link tissue cells experiencing intense mechanical stress. Although the structure of the desmosomal cadherins is known, the desmosome architecture-which is ...Desmosomes are cell-cell junctions that link tissue cells experiencing intense mechanical stress. Although the structure of the desmosomal cadherins is known, the desmosome architecture-which is essential for mediating numerous functions-remains elusive. Here, we recorded cryo-electron tomograms (cryo-ET) in which individual cadherins can be discerned; they appear variable in shape, spacing, and tilt with respect to the membrane. The resulting sub-tomogram average reaches a resolution of ∼26 Å, limited by the inherent flexibility of desmosomes. To address this challenge typical of dynamic biological assemblies, we combine sub-tomogram averaging with atomistic molecular dynamics (MD) simulations. We generate models of possible cadherin arrangements and perform an in silico screening according to biophysical and structural properties extracted from MD simulation trajectories. We find a truss-like arrangement of cadherins that resembles the characteristic footprint seen in the electron micrograph. The resulting model of the desmosomal architecture explains their unique biophysical properties and strength. |
リンク | Proc Natl Acad Sci U S A / PubMed:33067392 / PubMed Central |
手法 | EM (サブトモグラム平均) |
解像度 | 26.0 Å |
構造データ | EMDB-11678, PDB-7a7d: |
由来 |
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キーワード | CELL ADHESION / Cadherin / Desmosome |