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-Structure paper
| タイトル | Shotgun EM of mycobacterial protein complexes during stationary phase stress. |
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| ジャーナル・号・ページ | Curr Res Struct Biol, Vol. 2, Page 204-212, Year 2020 |
| 掲載日 | 2020年9月22日 |
著者 | Angela M Kirykowicz / Jeremy D Woodward / ![]() |
| PubMed 要旨 | There is little structural information about the protein complexes conferring resistance in to anti-microbial oxygen and nitrogen radicals in the phagolysosome. Here, we expose the model ...There is little structural information about the protein complexes conferring resistance in to anti-microbial oxygen and nitrogen radicals in the phagolysosome. Here, we expose the model Mycobacterium, to simulated oxidative-stress conditions and apply a shotgun EM method for the structural detection of the resulting protein assemblies. We identified: glutamine synthetase I, essential for virulence; bacterioferritin A, critical for iron regulation; aspartyl aminopeptidase M18, a protease; and encapsulin, which produces a cage-like structure to enclose cargo proteins. After further investigation, we found that encapsulin carries dye-decolourising peroxidase, a protein antioxidant, as its primary cargo under the conditions tested. |
リンク | Curr Res Struct Biol / PubMed:34235480 / PubMed Central |
| 手法 | EM (単粒子) |
| 解像度 | 16.0 - 27.0 Å |
| 構造データ | ![]() EMDB-11595: ![]() EMDB-11596: ![]() EMDB-11597: ![]() EMDB-11598: ![]() EMDB-11599: |
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Mycolicibacterium smegmatis MC2 155 (バクテリア)