EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Architecture and function of human uromodulin filaments in urinary tract infections.
ジャーナル・号・ページ	Science, Vol. 369, Issue 6506, Page 1005-1010, Year 2020
掲載日	2020年8月21日
著者	Gregor L Weiss / Jessica J Stanisich / Maximilian M Sauer / Chia-Wei Lin / Jonathan Eras / Dawid S Zyla / Johannes Trück / Olivier Devuyst / Markus Aebi / Martin Pilhofer / Rudi Glockshuber /
PubMed 要旨	Uromodulin is the most abundant protein in human urine, and it forms filaments that antagonize the adhesion of uropathogens; however, the filament structure and mechanism of protection remain poorly ...Uromodulin is the most abundant protein in human urine, and it forms filaments that antagonize the adhesion of uropathogens; however, the filament structure and mechanism of protection remain poorly understood. We used cryo-electron tomography to show that the uromodulin filament consists of a zigzag-shaped backbone with laterally protruding arms. N-glycosylation mapping and biophysical assays revealed that uromodulin acts as a multivalent ligand for the bacterial type 1 pilus adhesin, presenting specific epitopes on the regularly spaced arms. Imaging of uromodulin-uropathogen interactions in vitro and in patient urine showed that uromodulin filaments associate with uropathogens and mediate bacterial aggregation, which likely prevents adhesion and allows clearance by micturition. These results provide a framework for understanding uromodulin in urinary tract infections and in its more enigmatic roles in physiology and disease.
リンク	Science / PubMed:32616672
手法	EM (トモグラフィー) / EM (サブトモグラム平均)
解像度	15.6 - 17.3 Å
構造データ	EMDB-11128: Cryo-electron tomogram of human Uromodulin filaments 手法: EM (トモグラフィー) EMDB-11129: Subtomogram average of human Uromodulin filaments 手法: EM (サブトモグラム平均) / 解像度: 15.6 Å EMDB-11130: Cryo-electron tomogram of elastase treated human Uromodulin filaments (eUmod) 手法: EM (トモグラフィー) EMDB-11131: Subtomogram average of elastase treated human Uromodulin filaments (eUmod) 手法: EM (サブトモグラム平均) / 解像度: 17.3 Å EMDB-11132: Cryo-electron tomogram of human Uromodulin filaments incubated with FimH lectin domain 手法: EM (トモグラフィー) EMDB-11133: Subtomogram average of human Uromodulin filaments incubated with FimH lectin domain 手法: EM (サブトモグラム平均) / 解像度: 16.6 Å EMDB-11134: Cryo-electron tomogram of elastase treated human Uromodulin filaments incubated with FimH lectin domain 手法: EM (トモグラフィー) EMDB-11135: Subtomogram average of elastase treated human Uromodulin fibers incubated with FimH lectin domain 手法: EM (サブトモグラム平均) / 解像度: 15.9 Å EMDB-11136: Cryo-electron tomogram of E. coli AAEC [pSH2] incubated with human Uromodulin filaments 手法: EM (トモグラフィー) EMDB-11137: Cryo-electron tomogram of non-piliated E. coli AAEC189 incubated with human Uromodulin filaments 手法: EM (トモグラフィー) EMDB-11138: Cryo-electron tomogram of FIB milled E.coli - Uromodulin aggregates 手法: EM (トモグラフィー) EMDB-11139: Cryo-electron tomogram of E. coli AAEC189 [pSH2] incubated with elastase treated human Uromodulin filaments 手法: EM (トモグラフィー) EMDB-11140: Cryo-electron tomogram of urine from patient with E. coli urinary tract infection 手法: EM (トモグラフィー) EMDB-11141: Cryo-electron tomogram of urine from patient with K. pneumoniae urinary tract infection 手法: EM (トモグラフィー) EMDB-11142: Cryo-electron tomogram of urine from patient with P. aeruginosa urinary tract infection 手法: EM (トモグラフィー) EMDB-11143: Cryo-electron tomogram of urine from patient with S. mitis urinary tract infection 手法: EM (トモグラフィー)
由来	Homo sapiens (ヒト)