+検索条件
-Structure paper
タイトル | The native structure of the assembled matrix protein 1 of influenza A virus. |
---|---|
ジャーナル・号・ページ | Nature, Vol. 587, Issue 7834, Page 495-498, Year 2020 |
掲載日 | 2020年9月9日 |
著者 | Julia Peukes / Xiaoli Xiong / Simon Erlendsson / Kun Qu / William Wan / Leslie J Calder / Oliver Schraidt / Susann Kummer / Stefan M V Freund / Hans-Georg Kräusslich / John A G Briggs / |
PubMed 要旨 | Influenza A virus causes millions of severe cases of disease during annual epidemics. The most abundant protein in influenza virions is matrix protein 1 (M1), which mediates virus assembly by ...Influenza A virus causes millions of severe cases of disease during annual epidemics. The most abundant protein in influenza virions is matrix protein 1 (M1), which mediates virus assembly by forming an endoskeleton beneath the virus membrane. The structure of full-length M1, and how it oligomerizes to mediate the assembly of virions, is unknown. Here we determine the complete structure of assembled M1 within intact virus particles, as well as the structure of M1 oligomers reconstituted in vitro. We find that the C-terminal domain of M1 is disordered in solution but can fold and bind in trans to the N-terminal domain of another M1 monomer, thus polymerizing M1 into linear strands that coat the interior surface of the membrane of the assembling virion. In the M1 polymer, five histidine residues-contributed by three different monomers of M1-form a cluster that can serve as the pH-sensitive disassembly switch after entry into a target cell. These structures therefore reveal mechanisms of influenza virus assembly and disassembly. |
リンク | Nature / PubMed:32908308 / PubMed Central |
手法 | EM (トモグラフィー) / EM (サブトモグラム平均) / EM (らせん対称) |
解像度 | 3.8 - 8.0 Å |
構造データ | EMDB-11075: EMDB-11076: EMDB-11077: EMDB-11078: The structure of the matrix protein M1 from influenza A/Hong Kong/1/1968 VLPs (HA,NA,M1,M2) EMDB-11079: Helical reconstruction of influenza A virus M1 in complex with nucleic acid |
化合物 | ChemComp-HOH: |
由来 |
|
キーワード | VIRAL PROTEIN / Viral matrix protein / Membrane binding / pH Sensor / Polymer / M1 / Matrix protein / Influenza virus / Assembly / ribonucleoprotein complex |