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-Structure paper
タイトル | Structural Insights into the Mammalian Late-Stage Initiation Complexes. |
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ジャーナル・号・ページ | Cell Rep, Vol. 31, Issue 1, Page 107497, Year 2020 |
掲載日 | 2020年4月7日 |
著者 | Angelita Simonetti / Ewelina Guca / Anthony Bochler / Lauriane Kuhn / Yaser Hashem / |
PubMed 要旨 | In higher eukaryotes, the mRNA sequence in the direct vicinity of the start codon, called the Kozak sequence (CRCCaugG, where R is a purine), is known to influence the rate of the initiation process. ...In higher eukaryotes, the mRNA sequence in the direct vicinity of the start codon, called the Kozak sequence (CRCCaugG, where R is a purine), is known to influence the rate of the initiation process. However, the molecular basis underlying its role remains poorly understood. Here, we present the cryoelectron microscopy (cryo-EM) structures of mammalian late-stage 48S initiation complexes (LS48S ICs) in the presence of two different native mRNA sequences, β-globin and histone 4, at overall resolution of 3 and 3.5 Å, respectively. Our high-resolution structures unravel key interactions from the mRNA to eukaryotic initiation factors (eIFs): 1A, 2, 3, 18S rRNA, and several 40S ribosomal proteins. In addition, we are able to study the structural role of ABCE1 in the formation of native 48S ICs. Our results reveal a comprehensive map of ribosome/eIF-mRNA and ribosome/eIF-tRNA interactions and suggest the impact of mRNA sequence on the structure of the LS48S IC. |
リンク | Cell Rep / PubMed:32268096 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 3.0 - 14.3 Å |
構造データ | EMDB-10760, PDB-6yal: EMDB-10761, PDB-6yam: EMDB-10762, PDB-6yan: EMDB-10763: EMDB-10764: |
化合物 | ChemComp-SF4: ChemComp-MG: ChemComp-GNP: |
由来 |
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キーワード | TRANSLATION / initiation complex / 48S / eIF1A / eIF3 / ABCE1 / rabbit / translation initiation / eukaryotic initiation factor 1 / eukaryotic initiation factor 1A / eukaryotic initiation factor 3 / late-stage initiation complex |