+検索条件
-Structure paper
タイトル | Ribosome heterogeneity in Drosophila melanogaster gonads through paralog-switching. |
---|---|
ジャーナル・号・ページ | Nucleic Acids Res, Vol. 50, Issue 4, Page 2240-2257, Year 2022 |
掲載日 | 2022年2月28日 |
著者 | Tayah Hopes / Karl Norris / Michaela Agapiou / Charley G P McCarthy / Philip A Lewis / Mary J O'Connell / Juan Fontana / Julie L Aspden / |
PubMed 要旨 | Ribosomes have long been thought of as homogeneous macromolecular machines, but recent evidence suggests they are heterogeneous and could be specialised to regulate translation. Here, we have ...Ribosomes have long been thought of as homogeneous macromolecular machines, but recent evidence suggests they are heterogeneous and could be specialised to regulate translation. Here, we have characterised ribosomal protein heterogeneity across 4 tissues of Drosophila melanogaster. We find that testes and ovaries contain the most heterogeneous ribosome populations, which occurs through a combination of paralog-enrichment and paralog-switching. We have solved structures of ribosomes purified from in vivo tissues by cryo-EM, revealing differences in precise ribosomal arrangement for testis and ovary 80S ribosomes. Differences in the amino acid composition of paralog pairs and their localisation on the ribosome exterior indicate paralog-switching could alter the ribosome surface, enabling different proteins to regulate translation. One testis-specific paralog-switching pair is also found in humans, suggesting this is a conserved site of ribosome heterogeneity. Overall, this work allows us to propose that mRNA translation might be regulated in the gonads through ribosome heterogeneity, providing a potential means of ribosome specialisation. |
リンク | Nucleic Acids Res / PubMed:34283226 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 3.0 - 4.9 Å |
構造データ | EMDB-10622, PDB-6xu6: EMDB-10623, PDB-6xu7: EMDB-10624, PDB-6xu8: |
由来 |
|
キーワード | RIBOSOME / Specialised ribosome / Drosophila melanogaster / Testis / Ovary |