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-Structure paper
タイトル | Structural basis for RNA polymerase III transcription repression by Maf1. |
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ジャーナル・号・ページ | Nat Struct Mol Biol, Vol. 27, Issue 3, Page 229-232, Year 2020 |
掲載日 | 2020年2月17日 |
著者 | Matthias K Vorländer / Florence Baudin / Robyn D Moir / René Wetzel / Wim J H Hagen / Ian M Willis / Christoph W Müller / |
PubMed 要旨 | Maf1 is a conserved inhibitor of RNA polymerase III (Pol III) that influences phenotypes ranging from metabolic efficiency to lifespan. Here, we present a 3.3-Å-resolution cryo-EM structure of yeast ...Maf1 is a conserved inhibitor of RNA polymerase III (Pol III) that influences phenotypes ranging from metabolic efficiency to lifespan. Here, we present a 3.3-Å-resolution cryo-EM structure of yeast Maf1 bound to Pol III, establishing that Maf1 sequesters Pol III elements involved in transcription initiation and binds the mobile C34 winged helix 2 domain, sealing off the active site. The Maf1 binding site overlaps with that of TFIIIB in the preinitiation complex. |
リンク | Nat Struct Mol Biol / PubMed:32066962 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 3.25 Å |
構造データ | EMDB-10595, PDB-6tut: |
化合物 | ChemComp-ZN: |
由来 |
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キーワード | TRANSCRIPTION / RNA Polymerase III / Pol III / Maf1 / transcription inhibition |