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-Structure paper
タイトル | Symmetrical organization of proteins under docked synaptic vesicles. |
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ジャーナル・号・ページ | FEBS Lett, Vol. 593, Issue 2, Page 144-153, Year 2019 |
掲載日 | 2019年1月18日 |
![]() | Xia Li / Abhijith Radhakrishnan / Kirill Grushin / Ravikiran Kasula / Arunima Chaudhuri / Sujatha Gomathinayagam / Shyam S Krishnakumar / Jun Liu / James E Rothman / ![]() ![]() ![]() |
PubMed 要旨 | During calcium-regulated exocytosis, the constitutive fusion machinery is 'clamped' in a partially assembled state until synchronously released by calcium. The protein machinery involved in this ...During calcium-regulated exocytosis, the constitutive fusion machinery is 'clamped' in a partially assembled state until synchronously released by calcium. The protein machinery involved in this process is known, but the supra-molecular architecture and underlying mechanisms are unclear. Here, we use cryo-electron tomography analysis in nerve growth factor-differentiated neuro-endocrine (PC12) cells to delineate the organization of the release machinery under the docked vesicles. We find that exactly six exocytosis modules, each likely consisting of a single SNAREpin with its bound Synaptotagmins, Complexin, and Munc18 proteins, are symmetrically arranged at the vesicle-PM interface. Mutational analysis suggests that the symmetrical organization is templated by circular oligomers of Synaptotagmin. The observed arrangement, including its precise radial positioning, is in-line with the recently proposed 'buttressed ring hypothesis'. |
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手法 | EM (サブトモグラム平均) |
解像度 | 50.0 Å |
構造データ | ![]() EMDB-0413: ![]() EMDB-0414: |
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