EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Cryo-EM structures of the archaeal PAN-proteasome reveal an around-the-ring ATPase cycle.
ジャーナル・号・ページ	Proc Natl Acad Sci U S A, Vol. 116, Issue 2, Page 534-539, Year 2019
掲載日	2019年1月8日
著者	Parijat Majumder / Till Rudack / Florian Beck / Radostin Danev / Günter Pfeifer / István Nagy / Wolfgang Baumeister /
PubMed 要旨	Proteasomes occur in all three domains of life, and are the principal molecular machines for the regulated degradation of intracellular proteins. They play key roles in the maintenance of protein ...Proteasomes occur in all three domains of life, and are the principal molecular machines for the regulated degradation of intracellular proteins. They play key roles in the maintenance of protein homeostasis, and control vital cellular processes. While the eukaryotic 26S proteasome is extensively characterized, its putative evolutionary precursor, the archaeal proteasome, remains poorly understood. The primordial archaeal proteasome consists of a 20S proteolytic core particle (CP), and an AAA-ATPase module. This minimal complex degrades protein unassisted by non-ATPase subunits that are present in a 26S proteasome regulatory particle (RP). Using cryo-EM single-particle analysis, we determined structures of the archaeal CP in complex with the AAA-ATPase PAN (proteasome-activating nucleotidase). Five conformational states were identified, elucidating the functional cycle of PAN, and its interaction with the CP. Coexisting nucleotide states, and correlated intersubunit signaling features, coordinate rotation of the PAN-ATPase staircase, and allosterically regulate N-domain motions and CP gate opening. These findings reveal the structural basis for a sequential around-the-ring ATPase cycle, which is likely conserved in AAA-ATPases.
リンク	Proc Natl Acad Sci U S A / PubMed:30559193 / PubMed Central
手法	EM (単粒子)
解像度	3.69 - 7.04 Å
構造データ	0209 6he4 EMDB-0209, PDB-6he4: AAA-ATPase ring of PAN-proteasomes 手法: EM (単粒子) / 解像度: 4.85 Å 0210 6he5 EMDB-0210, PDB-6he5: 20S core particle of PAN-proteasomes 手法: EM (単粒子) / 解像度: 4.12 Å 0211 6he7 EMDB-0211, PDB-6he7: 20S proteasome from Archaeoglobus fulgidus 手法: EM (単粒子) / 解像度: 3.69 Å 0212 6he8 EMDB-0212, PDB-6he8: PAN-proteasome in state 1 手法: EM (単粒子) / 解像度: 6.86 Å 0213 6he9 EMDB-0213, PDB-6he9: PAN-proteasome in state 2 手法: EM (単粒子) / 解像度: 6.35 Å 0214 6hea EMDB-0214, PDB-6hea: PAN-proteasome in state 3 手法: EM (単粒子) / 解像度: 7.04 Å 0215 6hec EMDB-0215, PDB-6hec: PAN-proteasome in state 4 手法: EM (単粒子) / 解像度: 6.95 Å 0216 6hed EMDB-0216, PDB-6hed: PAN-proteasome in state 5 手法: EM (単粒子) / 解像度: 6.95 Å
化合物	ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM
由来	archaeoglobus fulgidus dsm 4304 (古細菌) archaeoglobus fulgidus (strain atcc 49558 / vc-16 / dsm 4304 / jcm 9628 / nbrc 100126) (古細菌)
キーワード	HYDROLASE / PAN / Proteasome / AAA-ATPase / Archaea