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-Structure paper
タイトル | Cryo-EM structure of the yeast respiratory supercomplex. |
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ジャーナル・号・ページ | Nat Struct Mol Biol, Vol. 26, Issue 1, Page 50-57, Year 2019 |
掲載日 | 2018年12月31日 |
![]() | Sorbhi Rathore / Jens Berndtsson / Lorena Marin-Buera / Julian Conrad / Marta Carroni / Peter Brzezinski / Martin Ott / ![]() |
PubMed 要旨 | Respiratory chain complexes execute energy conversion by connecting electron transport with proton translocation over the inner mitochondrial membrane to fuel ATP synthesis. Notably, these complexes ...Respiratory chain complexes execute energy conversion by connecting electron transport with proton translocation over the inner mitochondrial membrane to fuel ATP synthesis. Notably, these complexes form multi-enzyme assemblies known as respiratory supercomplexes. Here we used single-particle cryo-EM to determine the structures of the yeast mitochondrial respiratory supercomplexes IIIIV and IIIIV, at 3.2-Å and 3.5-Å resolutions, respectively. We revealed the overall architecture of the supercomplex, which deviates from the previously determined assemblies in mammals; obtained a near-atomic structure of the yeast complex IV; and identified the protein-protein and protein-lipid interactions implicated in supercomplex formation. Take together, our results demonstrate convergent evolution of supercomplexes in mitochondria that, while building similar assemblies, results in substantially different arrangements and structural solutions to support energy conversion. |
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手法 | EM (単粒子) |
解像度 | 3.23 - 3.5 Å |
構造データ | EMDB-0004, PDB-6giq: ![]() EMDB-0005: ![]() EMDB-0006: |
化合物 | ![]() ChemComp-6PH: ![]() ChemComp-HEM: ![]() ChemComp-8PE: ![]() ChemComp-CN5: ![]() ChemComp-UQ6: ![]() ChemComp-7PH: ![]() ChemComp-FES: ![]() ChemComp-PCF: ![]() ChemComp-9PE: ![]() ChemComp-CN3: ![]() ChemComp-CU: ![]() ChemComp-HEA: ![]() ChemComp-CUA: |
由来 |
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![]() | ELECTRON TRANSPORT / Respiratory chain / supercomplex / bc1 complex / cytochrome c oxidase |