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-Structure paper
タイトル | In vivo epidermal migration requires focal adhesion targeting of ACF7. |
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ジャーナル・号・ページ | Nat Commun, Vol. 7, Page 11692, Year 2016 |
掲載日 | 2016年5月24日 |
著者 | Jiping Yue / Yao Zhang / Wenguang G Liang / Xuewen Gou / Philbert Lee / Han Liu / Wanqing Lyu / Wei-Jen Tang / Shao-Yu Chen / Feng Yang / Hong Liang / Xiaoyang Wu / |
PubMed 要旨 | Turnover of focal adhesions allows cell retraction, which is essential for cell migration. The mammalian spectraplakin protein, ACF7 (Actin-Crosslinking Factor 7), promotes focal adhesion dynamics by ...Turnover of focal adhesions allows cell retraction, which is essential for cell migration. The mammalian spectraplakin protein, ACF7 (Actin-Crosslinking Factor 7), promotes focal adhesion dynamics by targeting of microtubule plus ends towards focal adhesions. However, it remains unclear how the activity of ACF7 is regulated spatiotemporally to achieve focal adhesion-specific guidance of microtubule. To explore the potential mechanisms, we resolve the crystal structure of ACF7's NT (amino-terminal) domain, which mediates F-actin interactions. Structural analysis leads to identification of a key tyrosine residue at the calponin homology (CH) domain of ACF7, whose phosphorylation by Src/FAK (focal adhesion kinase) complex is essential for F-actin binding of ACF7. Using skin epidermis as a model system, we further demonstrate that the phosphorylation of ACF7 plays an indispensable role in focal adhesion dynamics and epidermal migration in vitro and in vivo. Together, our findings provide critical insights into the molecular mechanisms underlying coordinated cytoskeletal dynamics during cell movement. |
リンク | Nat Commun / PubMed:27216888 / PubMed Central |
手法 | SAS (X-ray synchrotron) / X線回折 |
解像度 | 2.654 Å |
構造データ | SASDBL3: Highly similar to Actin cross-linking family protein 7 (ACF7) Homo Sapiens SASDBN3: Highly similar to Actin cross-linking family protein 7 (ACF7) Y259D mutant Homo Sapiens PDB-4z6g: |
化合物 | ChemComp-PO4: ChemComp-HOH: |
由来 |
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キーワード | CELL ADHESION / cytoskeleton / cell migration / microtubule / Focal adhesion |