EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Molecular basis of specific viral RNA recognition and 5'-end capping by the Chikungunya virus nsP1.
ジャーナル・号・ページ	Cell Rep, Vol. 40, Issue 4, Page 111133, Year 2022
掲載日	2022年7月26日
著者	Kuo Zhang / Michelle Cheok Yien Law / Trinh Mai Nguyen / Yaw Bia Tan / Melissa Wirawan / Yee-Song Law / Lak Shin Jeong / Dahai Luo /
PubMed 要旨	Many viruses encode RNA-modifying enzymes to edit the 5' end of viral RNA to mimic the cellular mRNA for effective protein translation, genome replication, and evasion of the host defense mechanisms. ...Many viruses encode RNA-modifying enzymes to edit the 5' end of viral RNA to mimic the cellular mRNA for effective protein translation, genome replication, and evasion of the host defense mechanisms. Alphavirus nsP1 synthesizes the 5' end Cap-0 structure of viral RNAs. However, the molecular basis of the capping process remains unclear. We determine high-resolution cryoelectron microscopy (cryo-EM) structures of Chikungunya virus nsP1 in complex with m7GTP/SAH, covalently attached m7GMP, and Cap-0 viral RNA. These structures reveal details of viral-RNA-capping reactions and uncover a sequence-specific virus RNA-recognition pattern that, in turn, regulates viral-RNA-capping efficiency to ensure optimal genome replication and subgenomic RNA transcription. This sequence-specific enzyme-RNA pairing is conserved across all alphaviruses.
リンク	Cell Rep / PubMed:35905713
手法	EM (単粒子)
解像度	2.18 - 2.62 Å
構造データ	31580 7fgg EMDB-31580, PDB-7fgg: Cryo-EM Structure of Chikungunya Virus Nonstructural Protein 1 with m7GTP 手法: EM (単粒子) / 解像度: 2.19 Å 31581 7fgh EMDB-31581, PDB-7fgh: Cryo-EM Structure of Chikungunya Virus Nonstructural Protein 1 with m7GMP 手法: EM (単粒子) / 解像度: 2.18 Å 31582 7fgi EMDB-31582, PDB-7fgi: Cryo-EM Structure of Chikungunya Virus Nonstructural Protein 1 with m7Gppp-AU 手法: EM (単粒子) / 解像度: 2.51 Å 32914 7x01 EMDB-32914, PDB-7x01: Cryo-EM Structure of Chikungunya Virus Nonstructural Protein 1 with inhibitor FHA 手法: EM (単粒子) / 解像度: 2.62 Å
化合物	ChemComp-ZN: Unknown entry ChemComp-SAH: S-ADENOSYL-L-HOMOCYSTEINE / S-アデノシルホモシステイン / S-アデノシル-L-ホモシステイン ChemComp-MGP: 7-METHYL-GUANOSINE-5'-TRIPHOSPHATE / 7-メチル-7-アゾニアグアノシン5′-三りん酸 ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-HOH: WATER / 水 ChemComp-G7M: N7-METHYL-GUANOSINE-5'-MONOPHOSPHATE / 7-メチルグアノシン-7-イウム5′-りん酸 ChemComp-GTA: P1-7-METHYLGUANOSINE-P3-ADENOSINE-5',5'-TRIPHOSPHATE / 7-メチルグアノシン-7-イウム5′-三りん酸O^γ-(5′-アデノシル) ChemComp-U: URIDINE-5'-MONOPHOSPHATE / UMP / ウリジル酸 ChemComp-G: GUANOSINE-5'-MONOPHOSPHATE / GMP / グアニル酸 ChemComp-7XQ: (1R,2S,3S,4R,5R)-3-(6-aminopurin-9-yl)-4-fluoranyl-5-(2-hydroxyethyl)cyclopentane-1,2-diol
由来	chikungunya virus strain s27-african prototype (チクングニア熱) chikungunya virus (strain s27-african prototype) (チクングニア熱)
キーワード	VIRAL PROTEIN (ウイルスタンパク質) / nonstructural protein (ウイルス非構造タンパク質) / Chikungunya virus (チクングニア熱) / RNA cap (5'キャップ) / replication (DNA複製) / inhibitor (酵素阻害剤)