EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Mycobacterial HelD is a nucleic acids-clearing factor for RNA polymerase.
ジャーナル・号・ページ	Nat Commun, Vol. 11, Issue 1, Page 6419, Year 2020
掲載日	2020年12月18日
著者	Tomáš Kouba / Tomáš Koval' / Petra Sudzinová / Jiří Pospíšil / Barbora Brezovská / Jarmila Hnilicová / Hana Šanderová / Martina Janoušková / Michaela Šiková / Petr Halada / Michal Sýkora / Ivan Barvík / Jiří Nováček / Mária Trundová / Jarmila Dušková / Tereza Skálová / URee Chon / Katsuhiko S Murakami / Jan Dohnálek / Libor Krásný /
PubMed 要旨	RNA synthesis is central to life, and RNA polymerase (RNAP) depends on accessory factors for recovery from stalled states and adaptation to environmental changes. Here, we investigated the mechanism ...RNA synthesis is central to life, and RNA polymerase (RNAP) depends on accessory factors for recovery from stalled states and adaptation to environmental changes. Here, we investigated the mechanism by which a helicase-like factor HelD recycles RNAP. We report a cryo-EM structure of a complex between the Mycobacterium smegmatis RNAP and HelD. The crescent-shaped HelD simultaneously penetrates deep into two RNAP channels that are responsible for nucleic acids binding and substrate delivery to the active site, thereby locking RNAP in an inactive state. We show that HelD prevents non-specific interactions between RNAP and DNA and dissociates stalled transcription elongation complexes. The liberated RNAP can either stay dormant, sequestered by HelD, or upon HelD release, restart transcription. Our results provide insights into the architecture and regulation of the highly medically-relevant mycobacterial transcription machinery and define HelD as a clearing factor that releases RNAP from nonfunctional complexes with nucleic acids.
リンク	Nat Commun / PubMed:33339823 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	2 - 3.36 Å
構造データ	10996 6yxu EMDB-10996, PDB-6yxu: Structure of Mycobacterium smegmatis HelD protein in complex with RNA polymerase core - State I, primary channel engaged 手法: EM (単粒子) / 解像度: 3.08 Å 11004 6yys EMDB-11004, PDB-6yys: Structure of Mycobacterium smegmatis HelD protein in complex with RNA polymerase core - State II, primary channel engaged and active site interfering 手法: EM (単粒子) / 解像度: 3.08 Å 11026 6z11 EMDB-11026, PDB-6z11: Structure of Mycobacterium smegmatis HelD protein in complex with RNA polymerase core - State III, primary channel dis-engaged and active site interfering 手法: EM (単粒子) / 解像度: 3.36 Å PDB-6vsx: X-ray crystal structure of the C-terminal domain of Bacillus subtilis RNA polymerase binding helicase HelD 手法: X-RAY DIFFRACTION / 解像度: 2.0 Å
化合物	ChemComp-PO4: PHOSPHATE ION / ホスファ-ト / リン酸塩 ChemComp-HOH: WATER / 水 ChemComp-ZN: Unknown entry ChemComp-MG: Unknown entry / マグネシウムジカチオン
由来	mycolicibacterium smegmatis mc2 155 (バクテリア) bacillus subtilis (枯草菌)
キーワード	TRANSCRIPTION (転写 (生物学)) / RNA polymerase (RNAポリメラーゼ) / Helicase (ヘリカーゼ) / transcription cycle helicase-like protein RNA polymerase / trasncription / transcription cycle helicase-like protein RNA polymerase transcription