+検索条件
-Structure paper
タイトル | Protein denaturation at the air-water interface and how to prevent it. |
---|---|
ジャーナル・号・ページ | Elife, Vol. 8, Year 2019 |
掲載日 | 2019年4月1日 |
著者 | Edoardo D'Imprima / Davide Floris / Mirko Joppe / Ricardo Sánchez / Martin Grininger / Werner Kühlbrandt / |
PubMed 要旨 | Electron cryo-microscopy analyzes the structure of proteins and protein complexes in vitrified solution. Proteins tend to adsorb to the air-water interface in unsupported films of aqueous solution, ...Electron cryo-microscopy analyzes the structure of proteins and protein complexes in vitrified solution. Proteins tend to adsorb to the air-water interface in unsupported films of aqueous solution, which can result in partial or complete denaturation. We investigated the structure of yeast fatty acid synthase at the air-water interface by electron cryo-tomography and single-particle image processing. Around 90% of complexes adsorbed to the air-water interface are partly denatured. We show that the unfolded regions face the air-water interface. Denaturation by contact with air may happen at any stage of specimen preparation. Denaturation at the air-water interface is completely avoided when the complex is plunge-frozen on a substrate of hydrophilized graphene. |
リンク | Elife / PubMed:30932812 / PubMed Central |
手法 | EM (単粒子) / EM (サブトモグラム平均) |
解像度 | 4.0 - 24.6 Å |
構造データ | EMDB-0178: EMDB-0179: |
由来 |
|