EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Germline VRC01 antibody recognition of a modified clade C HIV-1 envelope trimer and a glycosylated HIV-1 gp120 core.
ジャーナル・号・ページ	Elife, Vol. 7, Year 2018
掲載日	2018年11月7日
著者	Andrew J Borst / Connor E Weidle / Matthew D Gray / Brandon Frenz / Joost Snijder / M Gordon Joyce / Ivelin S Georgiev / Guillaume Be Stewart-Jones / Peter D Kwong / Andrew T McGuire / Frank DiMaio / Leonidas Stamatatos / Marie Pancera / David Veesler /
PubMed 要旨	VRC01 broadly neutralizing antibodies (bnAbs) target the CD4-binding site (CD4) of the human immunodeficiency virus-1 (HIV-1) envelope glycoprotein (Env). Unlike mature antibodies, corresponding ...VRC01 broadly neutralizing antibodies (bnAbs) target the CD4-binding site (CD4) of the human immunodeficiency virus-1 (HIV-1) envelope glycoprotein (Env). Unlike mature antibodies, corresponding VRC01 germline precursors poorly bind to Env. Immunogen design has mostly relied on glycan removal from trimeric Env constructs and has had limited success in eliciting mature VRC01 bnAbs. To better understand elicitation of such bnAbs, we characterized the inferred germline precursor of VRC01 in complex with a modified trimeric 426c Env by cryo-electron microscopy and a 426c gp120 core by X-ray crystallography, biolayer interferometry, immunoprecipitation, and glycoproteomics. Our results show VRC01 germline antibodies interacted with a wild-type 426c core lacking variable loops 1-3 in the presence and absence of a glycan at position Asn276, with the latter form binding with higher affinity than the former. Interactions in the presence of an Asn276 oligosaccharide could be enhanced upon carbohydrate shortening, which should be considered for immunogen design.
リンク	Elife / PubMed:30403372 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	2.315 - 4.8 Å
構造データ	9294 6myy EMDB-9294, PDB-6myy: Germline VRC01 antibody recognition of a modified clade C HIV-1 envelope trimer, 3 Fabs bound, sharpened map 手法: EM (単粒子) / 解像度: 3.8 Å EMDB-9295: Germline VRC01 antibody recognition of a modified clade C HIV-1 envelope trimer, 3 Fabs bound, unsharpened map 手法: EM (単粒子) / 解像度: 3.8 Å 9303 6mzj EMDB-9303, PDB-6mzj: Germline VRC01 antibody recognition of a modified clade C HIV-1 envelope trimer, 2 Fabs bound, sharpened map 手法: EM (単粒子) / 解像度: 4.8 Å EMDB-9304: Germline VRC01 antibody recognition of a modified clade C HIV-1 envelope trimer, 2 Fabs bound, unsharpened map 手法: EM (単粒子) / 解像度: 4.8 Å PDB-6mft: Crystal structure of glycosylated 426c HIV-1 gp120 core G459C in complex with glVRC01 A60C heavy chain 手法: X-RAY DIFFRACTION / 解像度: 2.315 Å
化合物	ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン / N-アセチルグルコサミン ChemComp-PEG: DI(HYDROXYETHYL)ETHER / ジエチレングリコ-ル / ジエチレングリコール ChemComp-CIT: CITRIC ACID / クエン酸 / クエン酸 ChemComp-EDO: 1,2-ETHANEDIOL / エチレングリコ-ル / エチレングリコール ChemComp-HOH: WATER / 水
由来	human immunodeficiency virus 1 (ヒト免疫不全ウイルス) homo sapiens (ヒト)
キーワード	IMMUNE SYSTEM/VIRAL PROTEIN (免疫系) / glycans (糖鎖) / germline / IMMUNE SYSTEM-VIRAL PROTEIN complex (免疫系) / VIRAL PROTEIN/IMMUNE SYSTEM (ウイルス性) / HIV envelope / germline VRC01 antibody / viral fusion protein / glycan shield / VIRAL PROTEIN-IMMUNE SYSTEM complex (ウイルス性)