EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Helicase-Dependent RNA Decay Illuminated by a Cryo-EM Structure of a Human Nuclear RNA Exosome-MTR4 Complex.
ジャーナル・号・ページ	Cell, Vol. 173, Issue 7, Page 1663-1677.e21, Year 2018
掲載日	2018年6月14日
著者	Eva-Maria Weick / M Rhyan Puno / Kurt Januszyk / John C Zinder / Michael A DiMattia / Christopher D Lima /
PubMed 要旨	The ribonucleolytic RNA exosome interacts with RNA helicases to degrade RNA. To understand how the 3' to 5' Mtr4 helicase engages RNA and the nuclear exosome, we reconstituted 14-subunit Mtr4- ...The ribonucleolytic RNA exosome interacts with RNA helicases to degrade RNA. To understand how the 3' to 5' Mtr4 helicase engages RNA and the nuclear exosome, we reconstituted 14-subunit Mtr4-containing RNA exosomes from Saccharomyces cerevisiae, Schizosaccharomyces pombe, and human and show that they unwind structured substrates to promote degradation. We loaded a human exosome with an optimized DNA-RNA chimera that stalls MTR4 during unwinding and determined its structure to an overall resolution of 3.45 Å by cryoelectron microscopy (cryo-EM). The structure reveals an RNA-engaged helicase atop the non-catalytic core, with RNA captured within the central channel and DIS3 exoribonuclease active site. MPP6 tethers MTR4 to the exosome through contacts to the RecA domains of MTR4. EXOSC10 remains bound to the core, but its catalytic module and cofactor C1D are displaced by RNA-engaged MTR4. Competition for the exosome core may ensure that RNA is committed to degradation by DIS3 when engaged by MTR4.
リンク	Cell / PubMed:29906447 / PubMed Central
手法	EM (単粒子)
解像度	3.31 - 8.22 Å
構造データ	7808 6d6q EMDB-7808, PDB-6d6q: Human nuclear exosome-MTR4 RNA complex - overall reconstruction 手法: EM (単粒子) / 解像度: 3.45 Å 7809 6d6r EMDB-7809, PDB-6d6r: Human nuclear exosome-MTR4 RNA complex - composite map after focused reconstruction 手法: EM (単粒子) / 解像度: 3.45 Å EMDB-7810: Human nuclear exosome-MTR4 RNA complex - focused reconstruction on the nine-subunit core, MPP6, and EXOSC10 手法: EM (単粒子) / 解像度: 3.31 Å EMDB-7812: Human nuclear exosome-MTR4 RNA complex - focused reconstruction on the DIS-PIN domain 手法: EM (単粒子) / 解像度: 3.4 Å EMDB-7813: Human nuclear exosome-MTR4 RNA complex - focused reconstruction on MTR4 手法: EM (単粒子) / 解像度: 3.54 Å EMDB-7814: Human nuclear exosome-MTR4 RNA complex - focused reconstruction on the DIS3-RNBCS domains 手法: EM (単粒子) / 解像度: 3.8 Å EMDB-7815: Human nuclear exosome-MTR4 RNA complex - focused reconstruction on MTR4-dsDNA/RNA class 手法: EM (単粒子) / 解像度: 3.91 Å EMDB-7818: Human nuclear exosome-MTR4 RNA complex - overall reconstruction - small class KOW domain open 手法: EM (単粒子) / 解像度: 7.75 Å EMDB-7819: Human nuclear exosome-MTR4 RNA complex - overall reconstruction - small class KOW domain closed 手法: EM (単粒子) / 解像度: 8.22 Å
化合物	ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ZN: Unknown entry ChemComp-ANP: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / AMP-PNP / AMP-PNP, エネルギー貯蔵分子類似体*YM
由来	homo sapiens (ヒト) synthetic construct (人工物)
キーワード	HYDROLASE (加水分解酵素) / RNA exosome / RNA degradation / ribonuclease (リボヌクレアーゼ) / helicase (ヘリカーゼ) / SF2 / RNA-protein complex / translocase (輸送酵素) / nuclear