EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Visualizing late states of human 40S ribosomal subunit maturation.
ジャーナル・号・ページ	Nature, Vol. 558, Issue 7709, Page 249-253, Year 2018
掲載日	2018年6月6日
著者	Michael Ameismeier / Jingdong Cheng / Otto Berninghausen / Roland Beckmann /
PubMed 要旨	The formation of eukaryotic ribosomal subunits extends from the nucleolus to the cytoplasm and entails hundreds of assembly factors. Despite differences in the pathways of ribosome formation, high- ...The formation of eukaryotic ribosomal subunits extends from the nucleolus to the cytoplasm and entails hundreds of assembly factors. Despite differences in the pathways of ribosome formation, high-resolution structural information has been available only from fungi. Here we present cryo-electron microscopy structures of late-stage human 40S assembly intermediates, representing one state reconstituted in vitro and five native states that range from nuclear to late cytoplasmic. The earliest particles reveal the position of the biogenesis factor RRP12 and distinct immature rRNA conformations that accompany the formation of the 40S subunit head. Molecular models of the late-acting assembly factors TSR1, RIOK1, RIOK2, ENP1, LTV1, PNO1 and NOB1 provide mechanistic details that underlie their contribution to a sequential 40S subunit assembly. The NOB1 architecture displays an inactive nuclease conformation that requires rearrangement of the PNO1-bound 3' rRNA, thereby coordinating the final rRNA folding steps with site 3 cleavage.
リンク	Nature / PubMed:29875412
手法	EM (単粒子)
解像度	3.5 - 4.5 Å
構造データ	4337 6g18 EMDB-4337, PDB-6g18: Cryo-EM structure of a late human pre-40S ribosomal subunit - State C 手法: EM (単粒子) / 解像度: 3.6 Å 4348 6g4s EMDB-4348, PDB-6g4s: Cryo-EM structure of a late human pre-40S ribosomal subunit - State B 手法: EM (単粒子) / 解像度: 4.0 Å 4349 6g4w EMDB-4349, PDB-6g4w: Cryo-EM structure of a late human pre-40S ribosomal subunit - State A 手法: EM (単粒子) / 解像度: 4.5 Å 4350 6g51 EMDB-4350, PDB-6g51: Cryo-EM structure of a late human pre-40S ribosomal subunit - State D 手法: EM (単粒子) / 解像度: 4.1 Å 4351 6g53 EMDB-4351, PDB-6g53: Cryo-EM structure of a late human pre-40S ribosomal subunit - State E 手法: EM (単粒子) / 解像度: 4.5 Å 4352 6g5h EMDB-4352, PDB-6g5h: Cryo-EM structure of a late human pre-40S ribosomal subunit - Mature 手法: EM (単粒子) / 解像度: 3.6 Å 4353 6g5i EMDB-4353, PDB-6g5i: Cryo-EM structure of a late human pre-40S ribosomal subunit - State R 手法: EM (単粒子) / 解像度: 3.5 Å
化合物	ChemComp-ZN: Unknown entry
由来	homo sapiens (ヒト) Human (ヒト)
キーワード	RIBOSOME (リボソーム) / 40S / pre-40S / ribosome biogenesis (リボソーム生合成)