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-Structure paper
タイトル | The complete structure of the small-subunit processome. |
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ジャーナル・号・ページ | Nat Struct Mol Biol, Vol. 24, Issue 11, Page 944-953, Year 2017 |
掲載日 | 2017年9月25日 |
著者 | Jonas Barandun / Malik Chaker-Margot / Mirjam Hunziker / Kelly R Molloy / Brian T Chait / Sebastian Klinge / |
PubMed 要旨 | The small-subunit processome represents the earliest stable precursor of the eukaryotic small ribosomal subunit. Here we present the cryo-EM structure of the Saccharomyces cerevisiae small-subunit ...The small-subunit processome represents the earliest stable precursor of the eukaryotic small ribosomal subunit. Here we present the cryo-EM structure of the Saccharomyces cerevisiae small-subunit processome at an overall resolution of 3.8 Å, which provides an essentially complete near-atomic model of this assembly. In this nucleolar superstructure, 51 ribosome-assembly factors and two RNAs encapsulate the 18S rRNA precursor and 15 ribosomal proteins in a state that precedes pre-rRNA cleavage at site A1. Extended flexible proteins are employed to connect distant sites in this particle. Molecular mimicry and steric hindrance, as well as protein- and RNA-mediated RNA remodeling, are used in a concerted fashion to prevent the premature formation of the central pseudoknot and its surrounding elements within the small ribosomal subunit. |
リンク | Nat Struct Mol Biol / PubMed:28945246 |
手法 | EM (単粒子) |
解像度 | 3.8 Å |
構造データ | |
化合物 | ChemComp-ZN: |
由来 |
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キーワード | RIBOSOME (リボソーム) / Ribosome assembly (リボソーム) |