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-Structure paper
タイトル | An Atomic Structure of the Human Spliceosome. |
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ジャーナル・号・ページ | Cell, Vol. 169, Issue 5, Page 918-929.e14, Year 2017 |
掲載日 | 2017年5月18日 |
著者 | Xiaofeng Zhang / Chuangye Yan / Jing Hang / Lorenzo I Finci / Jianlin Lei / Yigong Shi / |
PubMed 要旨 | Mechanistic understanding of pre-mRNA splicing requires detailed structural information on various states of the spliceosome. Here we report the cryo electron microscopy (cryo-EM) structure of the ...Mechanistic understanding of pre-mRNA splicing requires detailed structural information on various states of the spliceosome. Here we report the cryo electron microscopy (cryo-EM) structure of the human spliceosome just before exon ligation (the C complex) at an average resolution of 3.76 Å. The splicing factor Prp17 stabilizes the active site conformation. The step II factor Slu7 adopts an extended conformation, binds Prp8 and Cwc22, and is poised for selection of the 3'-splice site. Remarkably, the intron lariat traverses through a positively charged central channel of RBM22; this unusual organization suggests mechanisms of intron recruitment, confinement, and release. The protein PRKRIP1 forms a 100-Å α helix linking the distant U2 snRNP to the catalytic center. A 35-residue fragment of the ATPase/helicase Prp22 latches onto Prp8, and the quaternary exon junction complex (EJC) recognizes upstream 5'-exon sequences and associates with Cwc22 and the GTPase Snu114. These structural features reveal important mechanistic insights into exon ligation. |
リンク | Cell / PubMed:28502770 |
手法 | EM (単粒子) |
解像度 | 3.6 Å |
構造データ | |
化合物 | ChemComp-IHP: ChemComp-GTP: ChemComp-MG: ChemComp-ADP: ChemComp-ZN: ChemComp-ATP: |
由来 |
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キーワード | SPLICING / RNA splicing / human spliceosome (スプライセオソーム) / C* complex / atomic structure (原子) / step 2 factors / EJC |