EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural Basis of Backwards Motion in Kinesin-1-Kinesin-14 Chimera: Implication for Kinesin-14 Motility.
ジャーナル・号・ページ	Structure, Vol. 24, Issue 8, Page 1322-1334, Year 2016
掲載日	2016年8月2日
著者	Masahiko Yamagishi / Hideki Shigematsu / Takeshi Yokoyama / Masahide Kikkawa / Mitsuhiro Sugawa / Mari Aoki / Mikako Shirouzu / Junichiro Yajima / Ryo Nitta /
PubMed 要旨	Kinesin-14 is a unique minus-end-directed microtubule-based motor. A swinging motion of a class-specific N-terminal neck helix has been proposed to produce minus-end directionality. However, it is ...Kinesin-14 is a unique minus-end-directed microtubule-based motor. A swinging motion of a class-specific N-terminal neck helix has been proposed to produce minus-end directionality. However, it is unclear how swinging of the neck helix is driven by ATP hydrolysis utilizing the highly conserved catalytic core among all kinesins. Here, using a motility assay, we show that in addition to the neck helix, the conserved five residues at the C-terminal region in kinesin-14, namely the neck mimic, are necessary to give kinesin-1 an ability to reverse its directionality toward the minus end of microtubules. Our structural analyses further demonstrate that the C-terminal neck mimic, in cooperation with conformational changes in the catalytic core during ATP binding, forms a kinesin-14 bundle with the N-terminal neck helix to swing toward the minus end of microtubules. Thus, the neck mimic plays a crucial role in coupling the chemical ATPase reaction with the mechanical cycle to produce the minus-end-directed motility of kinesin-14.
リンク	Structure / PubMed:27452403
手法	EM (らせん対称)
解像度	5.8 - 6.6 Å
構造データ	8058 5hnw EMDB-8058, PDB-5hnw: Structural basis of backwards motion in kinesin-14: minus-end directed nKn664 in the AMPPNP state 手法: EM (らせん対称) / 解像度: 6.6 Å 8059 5hnx EMDB-8059, PDB-5hnx: Structural basis of backwards motion in kinesin-14: minus-end directed nKn664 in the nucleotide-free state 手法: EM (らせん対称) / 解像度: 6.6 Å 8060 5hny EMDB-8060, PDB-5hny: Structural basis of backwards motion in kinesin-14: plus-end directed nKn669 in the AMPPNP state 手法: EM (らせん対称) / 解像度: 6.3 Å 8061 5hnz EMDB-8061, PDB-5hnz: Structural basis of backwards motion in kinesin-14: plus-end directed nKn669 in the nucleotide-free state 手法: EM (らせん対称) / 解像度: 5.8 Å
化合物	ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-GTP: GUANOSINE-5'-TRIPHOSPHATE / GTP / GTP, エネルギー貯蔵分子YM / グアノシン三リン酸 ChemComp-GDP: GUANOSINE-5'-DIPHOSPHATE / GDP / GDP, エネルギー貯蔵分子YM / グアノシン二リン酸 ChemComp-TA1: TAXOL / パクリタキセル / 薬剤, 化学療法薬YM / パクリタキセル ChemComp-ANP: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / AMP-PNP / AMP-PNP, エネルギー貯蔵分子類似体YM
由来	rattus norvegicus (ドブネズミ) bos taurus (ウシ) drosophila melanogaster (キイロショウジョウバエ) Bovine (ウシ)
キーワード	TRANSPORT PROTEIN (運搬体タンパク質) / kinesin (キネシン) / kinesin-14 (キネシン) / microtubule (微小管) / ATPase (ATPアーゼ) / STRUCTURAL PROTEIN/MOTOR PROTEIN (構造) / STRUCTURAL PROTEIN-MOTOR PROTEIN complex (構造)