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-Structure paper
タイトル | The structure of a biologically active influenza virus ribonucleoprotein complex. |
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ジャーナル・号・ページ | PLoS Pathog, Vol. 5, Issue 6, Page e1000491, Year 2009 |
掲載日 | 2009年6月26日 |
著者 | Rocío Coloma / José M Valpuesta / Rocío Arranz / José L Carrascosa / Juan Ortín / Jaime Martín-Benito / |
PubMed 要旨 | The influenza viruses contain a segmented, single-stranded RNA genome of negative polarity. Each RNA segment is encapsidated by the nucleoprotein and the polymerase complex into ribonucleoprotein ...The influenza viruses contain a segmented, single-stranded RNA genome of negative polarity. Each RNA segment is encapsidated by the nucleoprotein and the polymerase complex into ribonucleoprotein particles (RNPs), which are responsible for virus transcription and replication. Despite their importance, information about the structure of these RNPs is scarce. We have determined the three-dimensional structure of a biologically active recombinant RNP by cryo-electron microscopy. The structure shows a nonameric nucleoprotein ring (at 12 Angstrom resolution) with two monomers connected to the polymerase complex (at 18 Angstrom resolution). Docking the atomic structures of the nucleoprotein and polymerase domains, as well as mutational analyses, has allowed us to define the interactions between the functional elements of the RNP and to propose the location of the viral RNA. Our results provide the first model for a functional negative-stranded RNA virus ribonucleoprotein complex. The structure reported here will serve as a framework to generate a quasi-atomic model of the molecular machine responsible for viral RNA synthesis and to test new models for virus RNA replication and transcription. |
リンク | PLoS Pathog / PubMed:19557158 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 12.0 Å |
構造データ | EMDB-1603: 12 Angstrom resolution cryo-electron microscopy reconstruction of a recombinant active ribonucleoprotein particle of influenza virus (9-fold symmetrized). |
由来 |
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キーワード | VIRAL PROTEIN (ウイルスタンパク質) / VIRAL NUCLEOPROTEIN / HOST-VIRUS INTERACTION / RNA VIRUSES / NUCLEOPROTEIN (核タンパク質) / RIBONUCLEOPROTEIN (核タンパク質) / RNA (リボ核酸) / VIRION (ウイルス) / NUCLEUS (細胞核) / INFLUENZA (インフルエンザ) / RNA-BINDING |