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-Structure paper
| タイトル | Structural basis of NINJ1-mediated plasma membrane rupture in cell death. |
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| ジャーナル・号・ページ | Nature, Vol. 618, Issue 7967, Page 1065-1071, Year 2023 |
| 掲載日 | 2023年5月17日 |
 著者 | Morris Degen / José Carlos Santos / Kristyna Pluhackova / Gonzalo Cebrero / Saray Ramos / Gytis Jankevicius / Ella Hartenian / Undina Guillerm / Stefania A Mari / Bastian Kohl / Daniel J Müller / Paul Schanda / Timm Maier / Camilo Perez / Christian Sieben / Petr Broz / Sebastian Hiller /    |
| PubMed 要旨 | Eukaryotic cells can undergo different forms of programmed cell death, many of which culminate in plasma membrane rupture as the defining terminal event. Plasma membrane rupture was long thought to ...Eukaryotic cells can undergo different forms of programmed cell death, many of which culminate in plasma membrane rupture as the defining terminal event. Plasma membrane rupture was long thought to be driven by osmotic pressure, but it has recently been shown to be in many cases an active process, mediated by the protein ninjurin-1 (NINJ1). Here we resolve the structure of NINJ1 and the mechanism by which it ruptures membranes. Super-resolution microscopy reveals that NINJ1 clusters into structurally diverse assemblies in the membranes of dying cells, in particular large, filamentous assemblies with branched morphology. A cryo-electron microscopy structure of NINJ1 filaments shows a tightly packed fence-like array of transmembrane α-helices. Filament directionality and stability is defined by two amphipathic α-helices that interlink adjacent filament subunits. The NINJ1 filament features a hydrophilic side and a hydrophobic side, and molecular dynamics simulations show that it can stably cap membrane edges. The function of the resulting supramolecular arrangement was validated by site-directed mutagenesis. Our data thus suggest that, during lytic cell death, the extracellular α-helices of NINJ1 insert into the plasma membrane to polymerize NINJ1 monomers into amphipathic filaments that rupture the plasma membrane. The membrane protein NINJ1 is therefore an interactive component of the eukaryotic cell membrane that functions as an in-built breaking point in response to activation of cell death. |
 リンク | Nature / PubMed:37198476 / PubMed Central |
| 手法 | EM (らせん対称) |
| 解像度 | 3.8 Å |
| 構造データ | EMDB-16799, PDB-8cqr: |
| 由来 |
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 キーワード |  MEMBRANE PROTEIN (膜タンパク質) / Polymer (重合体) / Cell death (細胞死) |
