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Structure paper

タイトル	Structural impact on SARS-CoV-2 spike protein by D614G substitution.
ジャーナル・号・ページ	Science, Vol. 372, Issue 6541, Page 525-530, Year 2021
掲載日	2021年4月30日
著者	Jun Zhang / Yongfei Cai / Tianshu Xiao / Jianming Lu / Hanqin Peng / Sarah M Sterling / Richard M Walsh / Sophia Rits-Volloch / Haisun Zhu / Alec N Woosley / Wei Yang / Piotr Sliz / Bing Chen /
PubMed 要旨	Substitution for aspartic acid (D) by glycine (G) at position 614 in the spike (S) protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) appears to facilitate rapid viral spread. ...Substitution for aspartic acid (D) by glycine (G) at position 614 in the spike (S) protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) appears to facilitate rapid viral spread. The G614 strain and its recent variants are now the dominant circulating forms. Here, we report cryo-electron microscopy structures of a full-length G614 S trimer, which adopts three distinct prefusion conformations that differ primarily by the position of one receptor-binding domain. A loop disordered in the D614 S trimer wedges between domains within a protomer in the G614 spike. This added interaction appears to prevent premature dissociation of the G614 trimer-effectively increasing the number of functional spikes and enhancing infectivity-and to modulate structural rearrangements for membrane fusion. These findings extend our understanding of viral entry and suggest an improved immunogen for vaccine development.
リンク	Science / PubMed:33727252 / PubMed Central
手法	EM (単粒子)
解像度	3.2 - 3.5 Å
構造データ	23010 7krq EMDB-23010, PDB-7krq: Structural impact on SARS-CoV-2 spike protein by D614G substitution 手法: EM (単粒子) / 解像度: 3.4 Å 23011 7krr EMDB-23011, PDB-7krr: Structural impact on SARS-CoV-2 spike protein by D614G substitution 手法: EM (単粒子) / 解像度: 3.5 Å 23012 7krs EMDB-23012, PDB-7krs: Structural impact on SARS-CoV-2 spike protein by D614G substitution 手法: EM (単粒子) / 解像度: 3.2 Å
化合物	ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン / N-アセチルグルコサミン
由来	severe acute respiratory syndrome coronavirus 2 (SARSコロナウイルス2)
キーワード	VIRAL PROTEIN (ウイルスタンパク質)