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Structure paper

タイトル	Cryo-EM reveals active site coordination within a multienzyme pre-rRNA processing complex.
ジャーナル・号・ページ	Nat Struct Mol Biol, Vol. 26, Issue 9, Page 830-839, Year 2019
掲載日	2019年9月5日
著者	Monica C Pillon / Allen L Hsu / Juno M Krahn / Jason G Williams / Kevin H Goslen / Mack Sobhany / Mario J Borgnia / Robin E Stanley /
PubMed 要旨	Ribosome assembly is a complex process reliant on the coordination of trans-acting enzymes to produce functional ribosomal subunits and secure the translational capacity of cells. The ...Ribosome assembly is a complex process reliant on the coordination of trans-acting enzymes to produce functional ribosomal subunits and secure the translational capacity of cells. The endoribonuclease (RNase) Las1 and the polynucleotide kinase (PNK) Grc3 assemble into a multienzyme complex, herein designated RNase PNK, to orchestrate processing of precursor ribosomal RNA (rRNA). RNase PNK belongs to the functionally diverse HEPN nuclease superfamily, whose members rely on distinct cues for nuclease activation. To establish how RNase PNK coordinates its dual enzymatic activities, we solved a series of cryo-EM structures of Chaetomium thermophilum RNase PNK in multiple conformational states. The structures reveal that RNase PNK adopts a butterfly-like architecture, harboring a composite HEPN nuclease active site flanked by discrete RNA kinase sites. We identify two molecular switches that coordinate nuclease and kinase function. Together, our structures and corresponding functional studies establish a new mechanism of HEPN nuclease activation essential for ribosome production.
リンク	Nat Struct Mol Biol / PubMed:31488907 / PubMed Central
手法	EM (単粒子)
解像度	2.9 - 3.2 Å
構造データ	20040 6of2 EMDB-20040, PDB-6of2: Precursor ribosomal RNA processing complex, State 2. 手法: EM (単粒子) / 解像度: 2.9 Å 20041 6of3 EMDB-20041, PDB-6of3: Precursor ribosomal RNA processing complex, State 1. 手法: EM (単粒子) / 解像度: 3.0 Å 20042 6of4 EMDB-20042, PDB-6of4: Precursor ribosomal RNA processing complex, apo-state. 手法: EM (単粒子) / 解像度: 3.2 Å
化合物	ChemComp-AGS: PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER / ATP-γ-S / ATP-gamma-S, エネルギー貯蔵分子類似体YM ChemComp-MG*: Unknown entry / マグネシウムジカチオン
由来	Chaetomium thermophilum (菌類) chaetomium thermophilum (strain dsm 1495 / cbs 144.50 / imi 039719) (菌類)
キーワード	RNA BINDING PROTEIN / Complex / Ribonuclease / Polynucleotide kinase