+
検索条件

キーワード
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

-
Structure paper

タイトル	Structural basis for transcription antitermination at bacterial intrinsic terminator.
ジャーナル・号・ページ	Nat Commun, Vol. 10, Issue 1, Page 3048, Year 2019
掲載日	2019年7月11日
著者	Linlin You / Jing Shi / Liqiang Shen / Lingting Li / Chengli Fang / Chengzhi Yu / Wenbo Cheng / Yu Feng / Yu Zhang /
PubMed 要旨	Bacteriophages typically hijack the host bacterial transcriptional machinery to regulate their own gene expression and that of the host bacteria. The structural basis for bacteriophage protein- ...Bacteriophages typically hijack the host bacterial transcriptional machinery to regulate their own gene expression and that of the host bacteria. The structural basis for bacteriophage protein-mediated transcription regulation-in particular transcription antitermination-is largely unknown. Here we report the 3.4 Å and 4.0 Å cryo-EM structures of two bacterial transcription elongation complexes (P7-NusA-TEC and P7-TEC) comprising the bacteriophage protein P7, a master host-transcription regulator encoded by bacteriophage Xp10 of the rice pathogen Xanthomonas oryzae pv. Oryzae (Xoo) and discuss the mechanisms by which P7 modulates the host bacterial RNAP. The structures together with biochemical evidence demonstrate that P7 prevents transcription termination by plugging up the RNAP RNA-exit channel and impeding RNA-hairpin formation at the intrinsic terminator. Moreover, P7 inhibits transcription initiation by restraining RNAP-clamp motions. Our study reveals the structural basis for transcription antitermination by phage proteins and provides insights into bacterial transcription regulation.
リンク	Nat Commun / PubMed:31296855 / PubMed Central
手法	EM (単粒子)
解像度	3.41 - 3.95 Å
構造データ	9785 6j9e EMDB-9785, PDB-6j9e: Cryo-EM structure of Xanthomonos oryzae transcription elongation complex with NusA and the bacteriophage protein P7 手法: EM (単粒子) / 解像度: 3.41 Å 9786 6j9f EMDB-9786, PDB-6j9f: Cryo-EM structure of Xanthomonos oryzae transcription elongation complex with the bacteriophage protein P7 手法: EM (単粒子) / 解像度: 3.95 Å
化合物	ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ZN: Unknown entry
由来	xanthomonas oryzae pv. oryzae (バクテリア) xanthomonas oryzae pv. oryzae (strain pxo99a) (バクテリア) xanthomonas oryzae pv. oryzae pxo99a (バクテリア) xanthomonas virus xp10 (ウイルス) synthetic construct (人工物) Xanthomonas oryzae (バクテリア) xanthomonas oryzae pv. oryzae maff 311018 (バクテリア)
キーワード	TRANSCRIPTION (転写 (生物学)) / RNA polymerase (RNAポリメラーゼ) / transcription termination / anti-termination / RNAP clamp / phage (ファージ) / transcription initiation (転写 (生物学)) / P7 / NusA / Xanthomonos oryzae / Xp10