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TitleMastigoneme structure reveals insights into the O-linked glycosylation code of native hydroxyproline-rich helices.
Journal, issue, pagesCell, Vol. 187, Issue 8, Page 1907-1921.e16, Year 2024
Publish dateMar 22, 2024
AuthorsJin Dai / Meisheng Ma / Qingwei Niu / Robyn J Eisert / Xiangli Wang / Poulomi Das / Karl F Lechtreck / Susan K Dutcher / Rui Zhang / Alan Brown /
PubMed AbstractHydroxyproline-rich glycoproteins (HRGPs) are a ubiquitous class of protein in the extracellular matrices and cell walls of plants and algae, yet little is known of their native structures or ...Hydroxyproline-rich glycoproteins (HRGPs) are a ubiquitous class of protein in the extracellular matrices and cell walls of plants and algae, yet little is known of their native structures or interactions. Here, we used electron cryomicroscopy (cryo-EM) to determine the structure of the hydroxyproline-rich mastigoneme, an extracellular filament isolated from the cilia of the alga Chlamydomonas reinhardtii. The structure demonstrates that mastigonemes are formed from two HRGPs (a filament of MST1 wrapped around a single copy of MST3) that both have hyperglycosylated poly(hydroxyproline) helices. Within the helices, O-linked glycosylation of the hydroxyproline residues and O-galactosylation of interspersed serine residues create a carbohydrate casing. Analysis of the associated glycans reveals how the pattern of hydroxyproline repetition determines the type and extent of glycosylation. MST3 possesses a PKD2-like transmembrane domain that forms a heteromeric polycystin-like cation channel with PKD2 and SIP, explaining how mastigonemes are tethered to ciliary membranes.
External linksCell / PubMed:38552624 / PubMed Central
MethodsEM (single particle)
Resolution3.1 Å
Structure data

EMDB-43889: Chlamydomonas reinhardtii mastigoneme (constituent map 1)
Method: EM (single particle) / Resolution: 3.1 Å

EMDB-43890: Chlamydomonas reinhardtii mastigoneme (constituent map 2)
Method: EM (single particle) / Resolution: 3.1 Å

EMDB-43891: Chlamydomonas reinhardtii mastigoneme (constituent map 3)
Method: EM (single particle) / Resolution: 3.1 Å

43892

9b4h

EMDB-43892: Composite cryo-EM map of the Chlamydomonas reinhardtii mastigoneme
PDB-9b4h: Chlamydomonas reinhardtii mastigoneme filament
Method: EM (single particle) / Resolution: 3.1 Å

Chemicals

PDB-1aio:
CRYSTAL STRUCTURE OF A DOUBLE-STRANDED DNA CONTAINING THE MAJOR ADDUCT OF THE ANTICANCER DRUG CISPLATIN

ChemComp-GLA:
alpha-D-galactopyranose

Source
  • chlamydomonas reinhardtii (plant)
KeywordsSTRUCTURAL PROTEIN / Mastigoneme / Glycosylated hydroxyproline

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