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TitleCryo-EM structures of the Spo11 core complex bound to DNA.
Journal, issue, pagesNat Struct Mol Biol, Year 2024
Publish dateSep 20, 2024
AuthorsYou Yu / Juncheng Wang / Kaixian Liu / Zhi Zheng / Meret Arter / Corentin Claeys Bouuaert / Stephen Pu / Dinshaw J Patel / Scott Keeney /
PubMed AbstractDNA double-strand breaks that initiate meiotic recombination are formed by the topoisomerase-relative enzyme Spo11, supported by conserved auxiliary factors. Because high-resolution structural data ...DNA double-strand breaks that initiate meiotic recombination are formed by the topoisomerase-relative enzyme Spo11, supported by conserved auxiliary factors. Because high-resolution structural data have not been available, many questions remain about the architecture of Spo11 and its partners and how they engage with DNA. We report cryo-electron microscopy structures at up to 3.3-Å resolution of DNA-bound core complexes of Saccharomyces cerevisiae Spo11 with Rec102, Rec104 and Ski8. In these structures, monomeric core complexes make extensive contacts with the DNA backbone and with the recessed 3'-OH and first 5' overhanging nucleotide, establishing the molecular determinants of DNA end-binding specificity and providing insight into DNA cleavage preferences in vivo. The structures of individual subunits and their interfaces, supported by functional data in yeast, provide insight into the role of metal ions in DNA binding and uncover unexpected structural variation in homologs of the Top6BL component of the core complex.
External linksNat Struct Mol Biol / PubMed:39304764
MethodsEM (single particle)
Resolution3.3 - 3.7 Å
Structure data

42497

8urq

EMDB-42497, PDB-8urq:
Spo11 core complex with gapped DNA
Method: EM (single particle) / Resolution: 3.3 Å

42501

8uru

EMDB-42501, PDB-8uru:
Spo11 core complex with hairpin DNA
Method: EM (single particle) / Resolution: 3.7 Å

Chemicals

ChemComp-MG:
Unknown entry

Source
  • saccharomyces cerevisiae s288c (yeast)
KeywordsDNA BINDING PROTEIN / Spo11 / Rec102 / Rec104 / Ski8 / DNA binding / Cross over.

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