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Title | Structural and antigenic characterization of the avian adeno-associated virus capsid. |
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Journal, issue, pages | J Virol, Vol. 97, Issue 10, Page e0078023, Year 2023 |
Publish date | Oct 31, 2023 |
Authors | Jane Hsi / Mario Mietzsch / Paul Chipman / Sandra Afione / Allison Zeher / Rick Huang / John Chiorini / Robert McKenna / |
PubMed Abstract | AAVs are extensively studied as promising therapeutic gene delivery vectors. In order to circumvent pre-existing antibodies targeting primate-based AAV capsids, the AAAV capsid was evaluated as an ...AAVs are extensively studied as promising therapeutic gene delivery vectors. In order to circumvent pre-existing antibodies targeting primate-based AAV capsids, the AAAV capsid was evaluated as an alternative to primate-based therapeutic vectors. Despite the high sequence diversity, the AAAV capsid was found to bind to a common glycan receptor, terminal galactose, which is also utilized by other AAVs already being utilized in gene therapy trials. However, contrary to the initial hypothesis, AAAV was recognized by approximately 30% of human sera tested. Structural and sequence comparisons point to conserved epitopes in the fivefold region of the capsid as the reason determinant for the observed cross-reactivity. |
External links | J Virol / PubMed:37702486 / PubMed Central |
Methods | EM (single particle) |
Resolution | 2.54 - 3.06 Å |
Structure data | EMDB-41208, PDB-8tex: EMDB-41209, PDB-8tey: |
Chemicals | ChemComp-D5M: |
Source |
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Keywords | VIRUS / Adeno-associated virus / capsid / quail bronchitis / avianAAV / AAAV |