Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Disease-specific tau filaments assemble via polymorphic intermediates.
Journal, issue, pages	Nature, Vol. 625, Issue 7993, Page 119-125, Year 2024
Publish date	Nov 29, 2023
Authors	Sofia Lövestam / David Li / Jane L Wagstaff / Abhay Kotecha / Dari Kimanius / Stephen H McLaughlin / Alexey G Murzin / Stefan M V Freund / Michel Goedert / Sjors H W Scheres /
PubMed Abstract	Intermediate species in the assembly of amyloid filaments are believed to play a central role in neurodegenerative diseases and may constitute important targets for therapeutic intervention. However, ...Intermediate species in the assembly of amyloid filaments are believed to play a central role in neurodegenerative diseases and may constitute important targets for therapeutic intervention. However, structural information about intermediate species has been scarce and the molecular mechanisms by which amyloids assemble remain largely unknown. Here we use time-resolved cryogenic electron microscopy to study the in vitro assembly of recombinant truncated tau (amino acid residues 297-391) into paired helical filaments of Alzheimer's disease or into filaments of chronic traumatic encephalopathy. We report the formation of a shared first intermediate amyloid filament, with an ordered core comprising residues 302-316. Nuclear magnetic resonance indicates that the same residues adopt rigid, β-strand-like conformations in monomeric tau. At later time points, the first intermediate amyloid disappears and we observe many different intermediate amyloid filaments, with structures that depend on the reaction conditions. At the end of both assembly reactions, most intermediate amyloids disappear and filaments with the same ordered cores as those from human brains remain. Our results provide structural insights into the processes of primary and secondary nucleation of amyloid assembly, with implications for the design of new therapies.
External links	Nature / PubMed:38030728 / PubMed Central
Methods	EM (helical sym.)
Resolution	1.75 - 21.1 Å
Structure data	17806 8ppo EMDB-17806, PDB-8ppo: First Intermediate Amyloid (FIA) - Tau Method: EM (helical sym.) / Resolution: 2.0 Å 18070 8q27 EMDB-18070, PDB-8q27: Tau: AD-MIA1 Method: EM (helical sym.) / Resolution: 2.02 Å 18109 8q2j EMDB-18109, PDB-8q2j: Tau - AD-MIA2 Method: EM (helical sym.) / Resolution: 2.23 Å 18111 8q2k EMDB-18111, PDB-8q2k: Tau - AD-MIA3 Method: EM (helical sym.) / Resolution: 2.88 Å 18112 8q2l EMDB-18112, PDB-8q2l: Tau - AD-MIA4 Method: EM (helical sym.) / Resolution: 2.2 Å 18215 8q7f EMDB-18215, PDB-8q7f: Tau - AD-MIA5 Method: EM (helical sym.) / Resolution: 3.72 Å 18219 8q7l EMDB-18219, PDB-8q7l: Tau - AD-MIA6 (tau intermediate amyloid) Method: EM (helical sym.) / Resolution: 2.82 Å 18224 8q7m EMDB-18224, PDB-8q7m: Tau - AD-MIA7 (tau intermediate amyloid) Method: EM (helical sym.) / Resolution: 3.26 Å 18228 8q7p EMDB-18228, PDB-8q7p: Tau - AD-MIA8 (intermediate amyloid) Method: EM (helical sym.) / Resolution: 3.28 Å 18233 8q7t EMDB-18233, PDB-8q7t: Tau - AD-MIA11 Method: EM (helical sym.) / Resolution: 3.0 Å 18249 8q88 EMDB-18249, PDB-8q88: Tau - AD-LIA2 (tau intermediate amyloid) Method: EM (helical sym.) / Resolution: 2.95 Å 18250 8q8c EMDB-18250, PDB-8q8c: Tau - AD-MIA10 (intermediate amyloid) Method: EM (helical sym.) / Resolution: 1.92 Å 18251 8q8d EMDB-18251, PDB-8q8d: Tau - AD-LIA6 (tau intermediate amyloid) Method: EM (helical sym.) / Resolution: 3.04 Å 18252 8q8e EMDB-18252, PDB-8q8e: Tau - AD-LIA4 (tau intermediate amyloid) Method: EM (helical sym.) / Resolution: 3.81 Å 18253 8q8f EMDB-18253, PDB-8q8f: Tau - AD-LIA5 (tau intermediate amyloid) Method: EM (helical sym.) / Resolution: 2.93 Å 18254 8q8l EMDB-18254, PDB-8q8l: Tau - AD-LIA7 (tau intermediate amyloid) Method: EM (helical sym.) / Resolution: 3.04 Å 18255 8q8m EMDB-18255, PDB-8q8m: Tau - AD-PHF long crossover Method: EM (helical sym.) / Resolution: 2.95 Å 18258 8q8r EMDB-18258, PDB-8q8r: Tau - AD-PHF Method: EM (helical sym.) / Resolution: 2.1 Å 18259 8q8s EMDB-18259, PDB-8q8s: Tau - AD-THF Method: EM (helical sym.) / Resolution: 2.68 Å 18261 8q8u EMDB-18261, PDB-8q8u: Tau - CTE-MIA1 (tau intermediate amyloid) Method: EM (helical sym.) / Resolution: 3.3 Å 18262 8q8v EMDB-18262, PDB-8q8v: Tau - CTE-MIA3 Method: EM (helical sym.) / Resolution: 3.8 Å 18263 8q8w EMDB-18263, PDB-8q8w: Tau - CTE-MIA4 (tau intermediate amyloid) Method: EM (helical sym.) / Resolution: 2.85 Å 18264 8q8x EMDB-18264, PDB-8q8x: Tau - CTE-MIA5 (tau intermediate amyloid) Method: EM (helical sym.) / Resolution: 2.54 Å 18265 8q8y EMDB-18265, PDB-8q8y: Tau - CTE-MIA6 (tau intermediate amyloid) Method: EM (helical sym.) / Resolution: 2.88 Å 18266 8q8z EMDB-18266, PDB-8q8z: Tau - CTE-MIA7 (tau intermediate amyloid) Method: EM (helical sym.) / Resolution: 3.16 Å 18270 8q97 EMDB-18270, PDB-8q97: Tau - CTE-MIA9 (tau intermediate amyloid) Method: EM (helical sym.) / Resolution: 2.99 Å 18271 8q98 EMDB-18271, PDB-8q98: Tau - CTE-MIA8 (tau intermediate amyloid) Method: EM (helical sym.) / Resolution: 1.75 Å 18272 8q99 EMDB-18272, PDB-8q99: Tau - CTE-MIA10 (tau intermediate amyloid) Method: EM (helical sym.) / Resolution: 2.7 Å 18273 8q9a EMDB-18273, PDB-8q9a: Tau - CTE-MIA11 (tau intermediate amyloid) Method: EM (helical sym.) / Resolution: 3.04 Å 18275 8q9b EMDB-18275, PDB-8q9b: Tau - CTE-MIA13 (tau intermediate amyloid) Method: EM (helical sym.) / Resolution: 3.1 Å 18276 8q9c EMDB-18276, PDB-8q9c: Tau - CTE-MIA14 (tau intermediate amyloid) Method: EM (helical sym.) / Resolution: 3.4 Å 18277 8q9d EMDB-18277, PDB-8q9d: Tau - CTE-MIA15 (tau intermediate amyloid) Method: EM (helical sym.) / Resolution: 3.16 Å 18278 8q9e EMDB-18278, PDB-8q9e: Tau - CTE-MIA18 (tau intermediate amyloid) Method: EM (helical sym.) / Resolution: 2.97 Å 18279 8q9f EMDB-18279, PDB-8q9f: Tau - CTE-LIA3 (tau intermediate amyloid) Method: EM (helical sym.) / Resolution: 1.91 Å 18280 8q9g EMDB-18280, PDB-8q9g: Tau - CTE-LIA5 (tau intermediate amyloid) Method: EM (helical sym.) / Resolution: 2.65 Å 18281 8q9h EMDB-18281, PDB-8q9h: Tau - CTE-LIA4 (tau intermediate amyloid) Method: EM (helical sym.) / Resolution: 2.18 Å 18282 8q9i EMDB-18282, PDB-8q9i: Tau - CTE-LIA6 (tau intermediate amyloid) Method: EM (helical sym.) / Resolution: 2.56 Å 18283 8q9j EMDB-18283, PDB-8q9j: Tau - CTE-LIA7 (tau intermediate amyloid) Method: EM (helical sym.) / Resolution: 2.96 Å 18284 8q9k EMDB-18284, PDB-8q9k: Tau - CTE-LIA13 (tau intermediate amyloid) Method: EM (helical sym.) / Resolution: 3.2 Å 18285 8q9l EMDB-18285, PDB-8q9l: Tau - CTE-LIA14 (tau intermediate amyloid) Method: EM (helical sym.) / Resolution: 2.76 Å 18286 8q9m EMDB-18286, PDB-8q9m: Tau - CTE-type I (tau intermediate amyloid) Method: EM (helical sym.) / Resolution: 2.65 Å 18287 8q9o EMDB-18287: Tau - CTE-type II (tau intermediate amyloid) PDB-8q9o: CTE tau intermediate amyloid (LIA-17) Method: EM (helical sym.) / Resolution: 3.1 Å 18331 8qcp EMDB-18331, PDB-8qcp: Tau - AD-LIA8 (tau intermediate amyloid) Method: EM (helical sym.) / Resolution: 3.21 Å 18333 8qcr EMDB-18333, PDB-8qcr: Tau - CTE-MIA12 (tau intermediate amyloid) Method: EM (helical sym.) / Resolution: 2.75 Å EMDB-18344: AD Tau intermediate amyloid (LIA-1) Method: EM (helical sym.) / Resolution: 7.2 Å EMDB-18347: AD Tau intermediate amyloid (LIA-3) Method: EM (helical sym.) / Resolution: 9.0 Å EMDB-18348: AD Tau intermediate amyloid (MIA-9) Method: EM (helical sym.) / Resolution: 7.34 Å EMDB-18349: AD Tau intermediate amyloid (QHF) Method: EM (helical sym.) / Resolution: 4.28 Å EMDB-18354: CTE Tau intermediate amyloid (MIA-2) Method: EM (helical sym.) / Resolution: 4.22 Å EMDB-18355: CTE Tau intermediate amyloid (MIA-16) Method: EM (helical sym.) / Resolution: 4.59 Å EMDB-18356: CTE Tau intermediate amyloid (MIA-19) Method: EM (helical sym.) / Resolution: 6.73 Å EMDB-18357: CTE Tau intermediate amyloid (MIA-20) Method: EM (helical sym.) / Resolution: 5.02 Å EMDB-18358: CTE Tau intermediate amyloid (LIA-1) Method: EM (helical sym.) / Resolution: 8.56 Å EMDB-18359: CTE Tau intermediate amyloid (LIA-2) Method: EM (helical sym.) / Resolution: 5.97 Å EMDB-18360: CTE Tau intermediate amyloid (LIA-8) Method: EM (helical sym.) / Resolution: 8.55 Å EMDB-18361: CTE Tau intermediate amyloid (LIA-9) Method: EM (helical sym.) / Resolution: 5.97 Å EMDB-18362: CTE Tau intermediate amyloid (LIA-10) Method: EM (helical sym.) / Resolution: 21.1 Å EMDB-18363: CTE Tau intermediate amyloid (LIA-11) Method: EM (helical sym.) / Resolution: 8.11 Å EMDB-18364: CTE Tau intermediate amyloid (LIA-12) Method: EM (helical sym.) / Resolution: 3.86 Å EMDB-18365: CTE Tau intermediate amyloid (LIA-15) Method: EM (helical sym.) / Resolution: 5.27 Å EMDB-18366: CTE Tau intermediate amyloid (LIA-16) Method: EM (helical sym.) / Resolution: 4.33 Å 18448 8qjj EMDB-18448, PDB-8qjj: CTE type II (tau intermediate amyloid) Method: EM (helical sym.) / Resolution: 3.35 Å
Source	homo sapiens (human)
Keywords	PROTEIN FIBRIL / Amyloid / tau