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Structure paper

TitleMolecular engineering of insulin for recombinant expression in yeast.
Journal, issue, pagesTrends Biotechnol, Vol. 42, Page 464-478, Year 2024
Publish dateJun 21, 2023 (structure data deposition date)
AuthorsKjeldsen, T. / Andersen, A.S. / Hubalek, F. / Johansson, E. / Kreiner, F.F. / Schluckebier, G. / Kurtzhals, P.
External linksTrends Biotechnol / PubMed:37880066
MethodsX-ray diffraction
Resolution1.25 - 2.14 Å
Structure data

PDB-8pi4:
Crystal structure of human insulin desB30 precursor with an Alanine-Methionine-Lysine C-peptide in dimer (T2) conformation
Method: X-RAY DIFFRACTION / Resolution: 1.25 Å

PDB-8pi5:
Crystal structure of human insulin desB30 precursor with an Alanine-Methionine-Lysine C-peptide in hexamer (T3R3) conformation
Method: X-RAY DIFFRACTION / Resolution: 1.66 Å

PDB-8pi6:
Crystal structure of the monomeric zinc free human insulin A22K, B3E, B26E, B29R, desB30 precursor with a Ser-Glu-Asp-Trp-Trp-Arg C-peptide and a Glu-Glu-Gly-Glu-Pro-Arg N-terminal extension
Method: X-RAY DIFFRACTION / Resolution: 2.14 Å

PDB-8pjc:
Crystal structure of human insulin desB30 precursor with an Alanine-Alanine-Lysine C-peptide in dimer (T2) conformation
Method: X-RAY DIFFRACTION / Resolution: 2.14 Å

PDB-8pjh:
Crystal structure of human insulin desB30 precursor with an Aspartate-Glycine-Lysine C-peptide in dimer (T2) conformation
Method: X-RAY DIFFRACTION / Resolution: 1.5 Å

Chemicals

ChemComp-HOH:
WATER

ChemComp-ZN:
Unknown entry

ChemComp-CL:
Unknown entry

ChemComp-RCO:
RESORCINOL

Source
  • homo sapiens (human)
KeywordsHORMONE / insulin / precursor / dimer

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