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TitleCryo-EM structures of tau filaments from SH-SY5Y cells seeded with brain extracts from cases of Alzheimer's disease and corticobasal degeneration.
Journal, issue, pagesFEBS Open Bio, Vol. 13, Issue 8, Page 1394-1404, Year 2023
Publish dateJul 7, 2023
AuthorsAiri Tarutani / Sofia Lövestam / Xianjun Zhang / Abhay Kotecha / Andrew C Robinson / David M A Mann / Yuko Saito / Shigeo Murayama / Taisuke Tomita / Michel Goedert / Sjors H W Scheres / Masato Hasegawa /
PubMed AbstractThe formation of amyloid filaments through templated seeding is believed to underlie the propagation of pathology in most human neurodegenerative diseases. A widely used model system to study this ...The formation of amyloid filaments through templated seeding is believed to underlie the propagation of pathology in most human neurodegenerative diseases. A widely used model system to study this process is to seed amyloid filament formation in cultured cells using human brain extracts. Here, we report the electron cryo-microscopy structures of tau filaments from  undifferentiated seeded SH-SY5Y cells that transiently expressed N-terminally HA-tagged 1N3R or 1N4R human tau, using brain extracts from individuals with Alzheimer's disease or corticobasal degeneration. Although the resulting filament structures differed from those of the brain seeds, some degrees of structural templating were observed. Studying templated seeding in cultured cells, and determining the structures of the resulting filaments, can thus provide insights into the cellular aspects underlying neurodegenerative diseases.
External linksFEBS Open Bio / PubMed:37337995 / PubMed Central
MethodsEM (helical sym.)
Resolution2.3 - 2.5 Å
Structure data

17121

8ore

EMDB-17121, PDB-8ore:
Cryo-EM structure of SH-SY5Y seeded with filaments from Alzheimer's Disease
Method: EM (helical sym.) / Resolution: 2.5 Å

17122

8orf

EMDB-17122, PDB-8orf:
Cryo-EM structure of SH-SY5Y seeded with filaments from corticobasal degeneration extracts
Method: EM (helical sym.) / Resolution: 2.5 Å

17123

8org

EMDB-17123, PDB-8org:
Cryo-EM structure of SH-SY5Y seeded with filaments from corticobasal degeneration extracts (Type II)
Method: EM (helical sym.) / Resolution: 2.3 Å

Source
  • homo sapiens (human)
KeywordsPROTEIN FIBRIL / Amyloid / tau

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