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-Structure paper
Title | Structural basis for sugar perception by gustatory receptors. |
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Journal, issue, pages | Science, Vol. 383, Issue 6685, Page eadj2609, Year 2024 |
Publish date | Feb 23, 2024 |
Authors | Demin Ma / Meiqin Hu / Xiaotong Yang / Qiang Liu / Fan Ye / Weijie Cai / Yong Wang / Ximing Xu / Shenghai Chang / Ruiying Wang / Wei Yang / Sheng Ye / Nannan Su / Minrui Fan / Haoxing Xu / Jiangtao Guo / |
PubMed Abstract | Insects rely on a family of seven transmembrane proteins called gustatory receptors (GRs) to encode different taste modalities, such as sweet and bitter. We report structures of sweet taste ...Insects rely on a family of seven transmembrane proteins called gustatory receptors (GRs) to encode different taste modalities, such as sweet and bitter. We report structures of sweet taste receptors GR43a and GR64a in the apo and sugar-bound states. Both GRs form tetrameric sugar-gated cation channels composed of one central pore domain (PD) and four peripheral ligand-binding domains (LBDs). Whereas GR43a is specifically activated by the monosaccharide fructose that binds to a narrow pocket in LBDs, disaccharides sucrose and maltose selectively activate GR64a by binding to a larger and flatter pocket in LBDs. Sugar binding to LBDs induces local conformational changes, which are subsequently transferred to the PD to cause channel opening. Our studies reveal a structural basis for sugar recognition and activation of GRs. |
External links | Science / PubMed:38305684 |
Methods | EM (single particle) |
Resolution | 2.5 - 3.1 Å |
Structure data | EMDB-36410, PDB-8jm9: EMDB-36411, PDB-8jma: EMDB-36417, PDB-8jme: EMDB-36421, PDB-8jmh: EMDB-36422, PDB-8jmi: EMDB-38133, PDB-8x82: EMDB-38134, PDB-8x83: EMDB-38135, PDB-8x84: |
Chemicals | ChemComp-FRU: ChemComp-NA: ChemComp-HOH: ChemComp-CA: |
Source |
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Keywords | MEMBRANE PROTEIN / gustatory receptor / Gr43a / ligand-gated ion channel / fructose / Gr64a / sucrose / maltose |