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TitleCryo-EM structure of a bacteriophage M13 mini variant.
Journal, issue, pagesNat Commun, Vol. 14, Issue 1, Page 5421, Year 2023
Publish dateSep 5, 2023
AuthorsQi Jia / Ye Xiang /
PubMed AbstractFilamentous bacteriophages package their circular, single stranded DNA genome with the major coat protein pVIII and the minor coat proteins pIII, pVII, pVI, and pIX. Here, we report the cryo-EM ...Filamentous bacteriophages package their circular, single stranded DNA genome with the major coat protein pVIII and the minor coat proteins pIII, pVII, pVI, and pIX. Here, we report the cryo-EM structure of a ~500 Å long bacteriophage M13 mini variant. The distal ends of the mini phage are sealed by two cap-like complexes composed of the minor coat proteins. The top cap complex consists of pVII and pIX, both exhibiting a single helix structure. Arg33 of pVII and Glu29 of pIX, located on the inner surface of the cap, play a key role in recognizing the genome packaging signal. The bottom cap complex is formed by the hook-like structures of pIII and pVI, arranged in helix barrels. Most of the inner ssDNA genome adopts a double helix structure with a similar pitch to that of the A-form double-stranded DNA. These findings provide insights into the assembly of filamentous bacteriophages.
External linksNat Commun / PubMed:37669979 / PubMed Central
MethodsEM (single particle)
Resolution3.1 - 3.9 Å
Structure data

35793

8ixj

EMDB-35793, PDB-8ixj:
Middle segment of the bacteriophage M13 mini variant
Method: EM (single particle) / Resolution: 3.1 Å

35794

8ixk

EMDB-35794, PDB-8ixk:
bottom segment of the bacteriophage M13 mini variant
Method: EM (single particle) / Resolution: 3.3 Å

35795

8ixl

EMDB-35795, PDB-8ixl:
top segment of the bacteriophage M13 mini variant
Method: EM (single particle) / Resolution: 3.5 Å

35796

8jwt

EMDB-35796: Asymmetric reconstruction of the middle segment of the bacteriophage M13 mini variant
PDB-8jwt: Asymmetric middle segment of the bacteriophage M13 mini variant
Method: EM (single particle) / Resolution: 3.4 Å

EMDB-35797: Asymmetric reconstruction of bottom segment of the bacteriophage M13 mini variant
Method: EM (single particle) / Resolution: 3.9 Å

EMDB-35798: Full length of the bacteriophage M13 mini variant
Method: EM (single particle) / Resolution: 3.5 Å

EMDB-35805: Asymmetric reconstruction of the full length of the bacteriophage M13 variant
Method: EM (single particle) / Resolution: 3.6 Å

Source
  • inovirus m13
  • enterobacteria phage m13 (virus)
KeywordsVIRAL PROTEIN / Viral coat protein / M13

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