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Title | Structural Basis for the Immunogenicity of the C-Terminus of VP1 of Echovirus 3 Revealed by the Binding of a Neutralizing Antibody. |
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Journal, issue, pages | Viruses, Vol. 14, Issue 11, Year 2022 |
Publish date | Oct 22, 2022 |
Authors | Shuai Qi / Wangjun Fu / Jinyan Fan / Li Zhang / Binyang Zheng / Kang Wang / Xiangxi Wang / Ling Zhu / Xinjian Li / Yuxia Zhang / |
PubMed Abstract | Echovirus 3 (E3), a serotype of human enterovirus B (HEV-B), causes severe diseases in infants. Here, we determined the structures of E3 with a monoclonal antibody (MAb) 6D10 by cryo-EM to ...Echovirus 3 (E3), a serotype of human enterovirus B (HEV-B), causes severe diseases in infants. Here, we determined the structures of E3 with a monoclonal antibody (MAb) 6D10 by cryo-EM to comprehensively understand the specificities and the immunological characteristic of this serotype. The solved cryo-EM structures of the F-, A-, and E-particles of E3 bound with 6D10 revealed the structural features of the virus-antibody interface. Importantly, the structures of E-particles bound with 6D10 revealed for the first time the nature of the C-terminus of VP1 for HEV-Bs at the structural level. The highly immunogenic nature of this region in the E-particles provides new strategies for vaccine development for HEV-Bs. |
External links | Viruses / PubMed:36366420 / PubMed Central |
Methods | EM (single particle) |
Resolution | 3.1 - 3.7 Å |
Structure data | EMDB-34231, PDB-8gsc: EMDB-34232, PDB-8gsd: EMDB-34233, PDB-8gse: EMDB-34234, PDB-8gsf: |
Chemicals | ChemComp-SPH: |
Source |
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Keywords | VIRUS/IMMUNE SYSTEM / E3 Full particle / Echovirus3 Full particle / antibody / 6D10 / VIRUS-IMMUNE SYSTEM complex / VIRAL PROTEIN/IMMUNE SYSTEM / VIRAL PROTEIN-IMMUNE SYSTEM complex |