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TitleΗigh-resolution structure of mammalian PI31-20S proteasome complex reveals mechanism of proteasome inhibition.
Journal, issue, pagesJ Biol Chem, Vol. 299, Issue 7, Page 104862, Year 2023
Publish dateMay 25, 2023
AuthorsHao-Chi Hsu / Jason Wang / Abbey Kjellgren / Huilin Li / George N DeMartino /
PubMed AbstractProteasome-catalyzed protein degradation mediates and regulates critical aspects of many cellular functions and is an important element of proteostasis in health and disease. Proteasome function is ...Proteasome-catalyzed protein degradation mediates and regulates critical aspects of many cellular functions and is an important element of proteostasis in health and disease. Proteasome function is determined in part by the types of proteasome holoenzymes formed between the 20S core particle that catalyzes peptide bond hydrolysis and any of multiple regulatory proteins to which it binds. One of these regulators, PI31, was previously identified as an in vitro 20S proteasome inhibitor, but neither the molecular mechanism nor the possible physiologic significance of PI31-mediated proteasome inhibition has been clear. Here we report a high-resolution cryo-EM structure of the mammalian 20S proteasome in complex with PI31. The structure shows that two copies of the intrinsically disordered carboxyl terminus of PI31 are present in the central cavity of the closed-gate conformation of the proteasome and interact with proteasome catalytic sites in a manner that blocks proteolysis of substrates but resists their own degradation. The two inhibitory polypeptide chains appear to originate from PI31 monomers that enter the catalytic chamber from opposite ends of the 20S cylinder. We present evidence that PI31 can inhibit proteasome activity in mammalian cells and may serve regulatory functions for the control of cellular proteostasis.
External linksJ Biol Chem / PubMed:37236357 / PubMed Central
MethodsEM (single particle)
Resolution2.23 - 2.54 Å
Structure data

29603

8fz5

EMDB-29603, PDB-8fz5:
The PI31-free Bovine 20S proteasome
Method: EM (single particle) / Resolution: 2.23 Å

29604

8fz6

EMDB-29604, PDB-8fz6:
The human PI31 complexed with bovine 20S proteasome
Method: EM (single particle) / Resolution: 2.54 Å

Source
  • bos taurus (cattle)
KeywordsHYDROLASE / bovine / proteasome / 20S / PI31 / inhibitor

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