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-Structure paper
Title | Molecular choreography of primer synthesis by the eukaryotic Pol α-primase. |
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Journal, issue, pages | Nat Commun, Vol. 14, Issue 1, Page 3697, Year 2023 |
Publish date | Jun 21, 2023 |
Authors | Zuanning Yuan / Roxana Georgescu / Huilin Li / Michael E O'Donnell / |
PubMed Abstract | The eukaryotic polymerase α (Pol α) synthesizes an RNA-DNA hybrid primer of 20-30 nucleotides. Pol α is composed of Pol1, Pol12, Primase 1 (Pri1), and Pri2. Pol1 and Pri1 contain the DNA ...The eukaryotic polymerase α (Pol α) synthesizes an RNA-DNA hybrid primer of 20-30 nucleotides. Pol α is composed of Pol1, Pol12, Primase 1 (Pri1), and Pri2. Pol1 and Pri1 contain the DNA polymerase and RNA primase activities, respectively. It has been unclear how Pol α hands over an RNA primer from Pri1 to Pol1 for DNA primer extension, and how the primer length is defined. Here we report the cryo-EM analysis of yeast Pol α in the apo, primer initiation, primer elongation, RNA primer hand-off from Pri1 to Pol1, and DNA extension states, revealing a series of very large movements. We reveal a critical point at which Pol1-core moves to take over the 3'-end of the RNA from Pri1. DNA extension is limited by a spiral motion of Pol1-core. Since both Pri1 and Pol1-core are flexibly attached to a stable platform, primer growth produces stress that limits the primer length. |
External links | Nat Commun / PubMed:37344454 / PubMed Central |
Methods | EM (single particle) |
Resolution | 3.56 - 5.6 Å |
Structure data | EMDB-29345, PDB-8foc: EMDB-29346, PDB-8fod: EMDB-29347, PDB-8foe: EMDB-29349, PDB-8foh: EMDB-29351, PDB-8foj: EMDB-29352, PDB-8fok: |
Chemicals | ChemComp-SF4: |
Source |
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Keywords | DNA BINDING PROTEIN / DNA polymerase / primase / DNA replication |