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TitleStructure and mechanism of sulfofructose transaldolase, a key enzyme in sulfoquinovose metabolism.
Journal, issue, pagesStructure, Vol. 31, Issue 3, Page 244-252.e4, Year 2023
Publish dateMar 2, 2023
AuthorsAlexander J D Snow / Mahima Sharma / Palika Abayakoon / Spencer J Williams / James N Blaza / Gideon J Davies /
PubMed AbstractSulfoquinovose (SQ) is a key component of plant sulfolipids (sulfoquinovosyl diacylglycerols) and a major environmental reservoir of biological sulfur. Breakdown of SQ is achieved by bacteria through ...Sulfoquinovose (SQ) is a key component of plant sulfolipids (sulfoquinovosyl diacylglycerols) and a major environmental reservoir of biological sulfur. Breakdown of SQ is achieved by bacteria through the pathways of sulfoglycolysis. The sulfoglycolytic sulfofructose transaldolase (sulfo-SFT) pathway is used by gut-resident firmicutes and soil saprophytes. After isomerization of SQ to sulfofructose (SF), the namesake enzyme catalyzes the transaldol reaction of SF transferring dihydroxyacetone to 3C/4C acceptors to give sulfolactaldehyde and fructose-6-phosphate or sedoheptulose-7-phosphate. We report the 3D cryo-EM structure of SF transaldolase from Bacillus megaterium in apo and ligand bound forms, revealing a decameric structure formed from two pentameric rings of the protomer. We demonstrate a covalent "Schiff base" intermediate formed by reaction of SF with Lys89 within a conserved Asp-Lys-Glu catalytic triad and defined by an Arg-Trp-Arg sulfonate recognition triad. The structural characterization of the signature enzyme of the sulfo-SFT pathway provides key insights into molecular recognition of the sulfonate group of sulfosugars.
External linksStructure / PubMed:36805128
MethodsEM (single particle) / X-ray diffraction
Resolution2.1 - 3.2 Å
Structure data

15960

8bc2

EMDB-15960, PDB-8bc2:
Ligand-Free Structure of the decameric sulfofructose transaldolase BmSF-TAL
Method: EM (single particle) / Resolution: 2.6 Å

15961

8bc3

EMDB-15961, PDB-8bc3:
Cryo-EM Structure of a BmSF-TAL - Sulfofructose Schiff Base Complex
Method: EM (single particle) / Resolution: 2.1 Å

15962

8bc4

EMDB-15962, PDB-8bc4:
Cryo-EM Structure of a BmSF-TAL - Sulfofructose Schiff Base Complex in symmetry group C1
Method: EM (single particle) / Resolution: 2.7 Å

PDB-8c4i:
Ligand-free Crystal Structure of the decameric Sulfofructose Transaldolase BmSF-TAL
Method: X-RAY DIFFRACTION / Resolution: 3.2 Å

Chemicals

ChemComp-HOH:
WATER

ChemComp-QC9:
(2~{R},3~{S},4~{S})-2,3,4,6-tetrakis(oxidanyl)hexane-1-sulfonic acid

Source
  • Priestia megaterium DSM 319 (bacteria)
  • bacillus aryabhattai (bacteria)
KeywordsTRANSFERASE / transaldolase / cryo-EM / decamer / sulfofructose / Sulfofugar

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