Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Conformational landscape of the type V-K CRISPR-associated transposon integration assembly.
Journal, issue, pages	Mol Cell, Vol. 84, Issue 12, Page 2353-22367.e5, Year 2024
Publish date	Jun 20, 2024
Authors	Francisco Tenjo-Castaño / Nicholas Sofos / Luisa S Stutzke / Piero Temperini / Anders Fuglsang / Tillmann Pape / Pablo Mesa / Guillermo Montoya /
PubMed Abstract	CRISPR-associated transposons (CASTs) are mobile genetic elements that co-opt CRISPR-Cas systems for RNA-guided DNA transposition. CASTs integrate large DNA cargos into the attachment (att) site ...CRISPR-associated transposons (CASTs) are mobile genetic elements that co-opt CRISPR-Cas systems for RNA-guided DNA transposition. CASTs integrate large DNA cargos into the attachment (att) site independently of homology-directed repair and thus hold promise for eukaryotic genome engineering. However, the functional diversity and complexity of CASTs hinder an understanding of their mechanisms. Here, we present the high-resolution cryoelectron microscopy (cryo-EM) structure of the reconstituted ∼1 MDa post-transposition complex of the type V-K CAST, together with different assembly intermediates and diverse TnsC filament lengths, thus enabling the recapitulation of the integration complex formation. The results of mutagenesis experiments probing the roles of specific residues and TnsB-binding sites show that transposition activity can be enhanced and suggest that the distance between the PAM and att sites is determined by the lengths of the TnsB C terminus and the TnsC filament. This singular model of RNA-guided transposition provides a foundation for repurposing the system for genome-editing applications.
External links	Mol Cell / PubMed:38834066
Methods	EM (single particle)
Resolution	2.3 - 3.19 Å
Structure data	15697 8axa EMDB-15697, PDB-8axa: Cryo-EM structure of shCas12k-sgRNA-dsDNA ternary complex (type V-K CRISPR-associated transposon) Method: EM (single particle) / Resolution: 2.96 Å 15698 8axb EMDB-15698, PDB-8axb: Cryo-EM structure of Cas12k-sgRNA binary complex (type V-K CRISPR-associated transposon) Method: EM (single particle) / Resolution: 2.87 Å 19075 8rdu EMDB-19075, PDB-8rdu: Conformational Landscape of the Type V-K CRISPR-associated TransposonIntegration Assembly CAST V-K composite map Method: EM (single particle) / Resolution: 2.3 Å 19282 8rkt EMDB-19282, PDB-8rkt: Conformational Landscape of the Type V-K CRISPR-associated TransposonIntegration Assembly CAST V-K Cas12k domain local-refinement map Method: EM (single particle) / Resolution: 2.35 Å 19283 8rku EMDB-19283, PDB-8rku: Conformational Landscape of the Type V-K CRISPR-associated TransposonIntegration Assembly CAST V-K TnsC domain local-refinement map Method: EM (single particle) / Resolution: 2.3 Å 19284 8rkv EMDB-19284, PDB-8rkv: Conformational Landscape of the Type V-K CRISPR-associated TransposonIntegration Assembly CAST V-K TnsB domain local-refinement map Method: EM (single particle) / Resolution: 3.11 Å EMDB-19286: consensus map of the V-K CRISPR-associated Transposon Integration Assembly Method: EM (single particle) / Resolution: 3.19 Å
Chemicals	ChemComp-HOH: WATER ChemComp-MG: Unknown entry ChemComp-ZN: Unknown entry ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM
Source	scytonema hofmannii (bacteria)
Keywords	DNA BINDING PROTEIN / CRISPR / complex / transposition / CRISPR-associated Transposon genome editing transposition