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Title | High-resolution reconstruction of a Jumbo-bacteriophage infecting capsulated bacteria using hyperbranched tail fibers. |
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Journal, issue, pages | Nat Commun, Vol. 13, Issue 1, Page 7241, Year 2022 |
Publish date | Nov 24, 2022 |
Authors | Ruochen Ouyang / Ana Rita Costa / C Keith Cassidy / Aleksandra Otwinowska / Vera C J Williams / Agnieszka Latka / Phill J Stansfeld / Zuzanna Drulis-Kawa / Yves Briers / Daniël M Pelt / Stan J J Brouns / Ariane Briegel / |
PubMed Abstract | The Klebsiella jumbo myophage ϕKp24 displays an unusually complex arrangement of tail fibers interacting with a host cell. In this study, we combine cryo-electron microscopy methods, protein ...The Klebsiella jumbo myophage ϕKp24 displays an unusually complex arrangement of tail fibers interacting with a host cell. In this study, we combine cryo-electron microscopy methods, protein structure prediction methods, molecular simulations, microbiological and machine learning approaches to explore the capsid, tail, and tail fibers of ϕKp24. We determine the structure of the capsid and tail at 4.1 Å and 3.0 Å resolution. We observe the tail fibers are branched and rearranged dramatically upon cell surface attachment. This complex configuration involves fourteen putative tail fibers with depolymerase activity that provide ϕKp24 with the ability to infect a broad panel of capsular polysaccharide (CPS) types of Klebsiella pneumoniae. Our study provides structural and functional insight into how ϕKp24 adapts to the variable surfaces of capsulated bacterial pathogens, which is useful for the development of phage therapy approaches against pan-drug resistant K. pneumoniae strains. |
External links | Nat Commun / PubMed:36433970 / PubMed Central |
Methods | EM (single particle) / EM (helical sym.) |
Resolution | 3.0 - 4.3 Å |
Structure data | EMDB-13862: Jumbo Phage phi-Kp24 empty capsid EMDB-14356: Jumbo Phage phi-Kp24 full capsid EMDB-14357: Jumbo Phage phi-Kp24 extended tail |
Source |
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Keywords | STRUCTURAL PROTEIN / Jumbo Phage / Klebsiella pneumoniae / tail / sheath / VIRUS / Klebsiella pheumoniae / Capsid |